Talk:20.109(F13): Mod 2 Day 6 Analysis & Planning 2

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(TR Blots)
Current revision (12:17, 6 November 2013) (view source)
 
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===TR Blots===
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===TR Blots===
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[[Image:Yellow_TR_EGFR.tif|thumb|left|300px|Yellow EGFR]]
+
'''TR Intensities'''
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[[Image:Yellow_TR_ERK.tif|thumb|left|300px|Yellow ERK]]
+
Defaults
 +
*EGFR - 700:5, 800:3
 +
*GAPDH - 700:2, 800:5
 +
*Akt - 700:2, 800: 6
 +
*ERK - 700:1, 800:3
 +
*Pink STAT3 - 700:7, 800:9
 +
*Pink EGFR - 700:7, 800:3
 +
[[Image:Yellow_TR_EGFR.tif|thumb|left|300px|Yellow EGFR]]  
 +
[[Image:Yellow_TR_ERK.tif|thumb|left|300px|Yellow ERK]]
 +
[[Image:Purple_TR_EGFR.tif|thumb|left|300px|Purple EGFR]]
 +
[[Image:Purple_TR_ERK.tif|thumb|left|300px|Purple ERK]]
[[Image:White_TR_EGFR.tif|thumb|left|300px|White EGFR]]
[[Image:White_TR_EGFR.tif|thumb|left|300px|White EGFR]]
[[Image:White_TR_ERK.tif|thumb|left|300px|White ERK]]
[[Image:White_TR_ERK.tif|thumb|left|300px|White ERK]]
Line 7: Line 17:
[[Image:Green_TR_pAkt.tif|thumb|left|300px|Green pAkt]]
[[Image:Green_TR_pAkt.tif|thumb|left|300px|Green pAkt]]
[[Image:Green_TR_GAPDH.tif|thumb|left|300px|Green GAPDH]]
[[Image:Green_TR_GAPDH.tif|thumb|left|300px|Green GAPDH]]
 +
[[Image:Green_TR_pAkt2.tif|thumb|left|300px|Green pAkt_2]]
 +
[[Image:Green_TR_GAPDH2.tif|thumb|left|300px|Green GAPDH_2]]
 +
[[Image:Green_TR_totalAkt2.tif|thumb|left|300px|Green Total Akt 2]]
[[Image:Orange_TR_EGFR.tif|thumb|left|300px|Orange EGFR]]
[[Image:Orange_TR_EGFR.tif|thumb|left|300px|Orange EGFR]]
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[[Image:Orange_TR_pAkt.tif|thumb|left|300px|Orange pAkt]]
+
[[Image:Orange_pAkt.tif|thumb|left|300px|Orange pAkt]]
[[Image:Orange_TR_GAPDH.tif|thumb|left|300px|Orange GAPDH]]
[[Image:Orange_TR_GAPDH.tif|thumb|left|300px|Orange GAPDH]]
 +
[[Image:Orange_TR_pAkt2.tif|thumb|left|300px|Orange pAkt_2]]
 +
[[Image:Orange_TR_GAPDH2.tif|thumb|left|300px|Orange GAPDH_2]]
 +
[[Image:Orange_TR_totalAkt2.tif|thumb|left|300px|Orange Total Akt 2]]
[[Image:Pink_TR_EGFR2.tif|thumb|left|300px|Pink EGFR]]
[[Image:Pink_TR_EGFR2.tif|thumb|left|300px|Pink EGFR]]
[[Image:Pink_TR_STAT3.tif|thumb|left|300px|Pink STAT3]]
[[Image:Pink_TR_STAT3.tif|thumb|left|300px|Pink STAT3]]
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[[Image:Blue_TR_STAT3.tif|thumb|left|300px|Blue STAT3]]
[[Image:Blue_TR_STAT3.tif|thumb|left|300px|Blue STAT3]]
[[Image:Blue_TR_GAPDH.tif|thumb|left|300px|Blue GAPDH]]
[[Image:Blue_TR_GAPDH.tif|thumb|left|300px|Blue GAPDH]]
 +
[[Image:Red_TR_EGFR.tif|thumb|left|300px|Red EGFR]]
 +
[[Image:Red_TR_STAT3.tif|thumb|left|300px|Red STAT3]]
 +
[[Image:Red_TR_GAPDH.tif|thumb|left|300px|Red GAPDH]]
 +
[[Image:Green_EGFR.tif|thumb|left|300px|Green EGFR]]
 +
[[Image:Green_pAkt.tif|thumb|left|300px|Green pAkt]]
 +
[[Image:Green_GAPDH.tif|thumb|left|300px|Green GAPDH]]
 +
[[Image:Plat_EGFR.tif|thumb|left|300px|Plat EGFR]]
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[[Image:Plat_pAkt.tif|thumb|left|300px|Plat pAkt]]
 +
[[Image:Plat_GAPDH.tif|thumb|left|300px|Plat GAPDH]]
 +
[[Image:Plat_TR_pAkt2.tif|thumb|left|300px|Plat pAkt_2]]
 +
[[Image:Plat_TR_GAPDH2.tif|thumb|left|300px|Plat GAPDH_2]]
 +
[[Image:Plat_TR_totalAkt2.tif|thumb|left|300px|Plat Total Akt 2]]
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<br style="clear:both;"/>
 +
 +
===WF Blots===
 +
'''WF Intensities'''
 +
ERK
 +
*White: 2/3
 +
*Pink: 1.5/3
 +
*Green: 2.5/3
 +
STAT3
 +
*Red: 6/7.5
 +
*Blue: 6/8
 +
GAPDH (All Teams)
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* 2/5
 +
[[Image:Yellow_WF_EGFR.tif|thumb|center|300px|Yellow EGFR]]
 +
[[Image:Yellow_WF_pAkt.tif|thumb|center|300px|Yellow pAkt]]
 +
[[Image:Yellow_WF_GAPDH.tif|thumb|center|300px|Yellow GAPDH]]
 +
[[Image:Yellow_WF_totalAkt.tif|thumb|center|300px|Yellow total Akt]]
 +
*Note, Yellow blots are in the reverse order.
 +
[[Image:Purple_WF_EGFR.tif|thumb|center|300px|Purple EGFR]]
 +
[[Image:Purple_pAkt.tif|thumb|center|300px|Purple pAkt]]
 +
[[Image:Purple_WF_GAPDH.tif|thumb|center|300px|Purple GAPDH]]
 +
[[Image:Orange_WF_EGFR.tif|thumb|center|300px|Orange EGFR]]
 +
[[Image:Orange_WF_GAPDH.tif|thumb|center|300px|Orange GAPDH]]
 +
[[Image:Red_WF_EGFR.tif|thumb|center|300px|Red EGFR]]
 +
[[Image:Red_WF_STAT3_2.tif|thumb|center|300px|Red STAT3]]
 +
[[Image:Red_WF_GAPDH.tif|thumb|center|300px|Red GAPDH]]
 +
[[Image:Blue_WF_EGFR.tif|thumb|center|300px|Blue EGFR]]
 +
[[Image:Blue_WF_STAT3_2.tif|thumb|center|300px|Blue STAT3]]
 +
[[Image:Blue_WF_GAPDH.tif|thumb|center|300px|Blue GAPDH]]
 +
[[Image:Pink_WF_EGFR.tif|thumb|center|300px|Pink EGFR]]
 +
[[Image:Pink_WF_ERK.tif|thumb|center|300px|Pink ERK]]
 +
[[Image:Green_WF_EGFR.tif|thumb|center|300px|Green EGFR]]
 +
[[Image:Green_WF_ERK.tif|thumb|center|300px|Green ERK]]
 +
[[Image:White_WF_EGFR.tif|thumb|center|300px|White EGFR]]
 +
[[Image:White_WF_Erk.tif|thumb|center|300px|White ERK]]

Current revision

TR Blots

TR Intensities Defaults

  • EGFR - 700:5, 800:3
  • GAPDH - 700:2, 800:5
  • Akt - 700:2, 800: 6
  • ERK - 700:1, 800:3
  • Pink STAT3 - 700:7, 800:9
  • Pink EGFR - 700:7, 800:3
Yellow EGFR
Yellow EGFR
Yellow ERK
Yellow ERK
Purple EGFR
Purple EGFR
Purple ERK
Purple ERK
White EGFR
White EGFR
White ERK
White ERK
Green EGFR
Green EGFR
Green pAkt
Green pAkt
Green GAPDH
Green GAPDH
Green pAkt_2
Green pAkt_2
Green GAPDH_2
Green GAPDH_2
Green Total Akt 2
Green Total Akt 2
Orange EGFR
Orange EGFR
Orange pAkt
Orange pAkt
Orange GAPDH
Orange GAPDH
Orange pAkt_2
Orange pAkt_2
Orange GAPDH_2
Orange GAPDH_2
Orange Total Akt 2
Orange Total Akt 2
Pink EGFR
Pink EGFR
Pink STAT3
Pink STAT3
Pink GAPDH
Pink GAPDH
Blue EGFR
Blue EGFR
Blue STAT3
Blue STAT3
Blue GAPDH
Blue GAPDH
Red EGFR
Red EGFR
Red STAT3
Red STAT3
Red GAPDH
Red GAPDH
Green EGFR
Green EGFR
Green pAkt
Green pAkt
Green GAPDH
Green GAPDH
Plat EGFR
Plat EGFR
Plat pAkt
Plat pAkt
Plat GAPDH
Plat GAPDH
Plat pAkt_2
Plat pAkt_2
Plat GAPDH_2
Plat GAPDH_2
Plat Total Akt 2
Plat Total Akt 2


WF Blots

WF Intensities ERK

  • White: 2/3
  • Pink: 1.5/3
  • Green: 2.5/3

STAT3

  • Red: 6/7.5
  • Blue: 6/8

GAPDH (All Teams)

  • 2/5
Yellow EGFR
Yellow EGFR
Yellow pAkt
Yellow pAkt
Yellow GAPDH
Yellow GAPDH
Yellow total Akt
Yellow total Akt
  • Note, Yellow blots are in the reverse order.
Purple EGFR
Purple EGFR
Purple pAkt
Purple pAkt
Purple GAPDH
Purple GAPDH
Orange EGFR
Orange EGFR
Orange GAPDH
Orange GAPDH
Red EGFR
Red EGFR
Red STAT3
Red STAT3
Red GAPDH
Red GAPDH
Blue EGFR
Blue EGFR
Blue STAT3
Blue STAT3
Blue GAPDH
Blue GAPDH
Pink EGFR
Pink EGFR
Pink ERK
Pink ERK
Green EGFR
Green EGFR
Green ERK
Green ERK
White EGFR
White EGFR
White ERK
White ERK
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