Talk:20.109(S07): Start-up expression engineering
From OpenWetWare
(Difference between revisions)
| Line 1: | Line 1: | ||
| + | On Day 1 of Module 3, you did a few things: | ||
| + | # Learned about the SAGA chromatin-remodeling complex and chose a non-essential sub-unit of the SAGA | ||
| + | complex to delet | ||
| + | # Designed primers with landing se | ||
| + | |||
[[Image:Gel mod3day1 PCR products.JPG]] | [[Image:Gel mod3day1 PCR products.JPG]] | ||
| + | |||
| + | ==Gel lane loading order== | ||
| + | |||
| + | ===Top row, Tue/Thur Section Samples=== | ||
# empty | # empty | ||
| Line 9: | Line 18: | ||
# red team +template, good | # red team +template, good | ||
# red team -template, good | # red team -template, good | ||
| + | # pink team -template, contaminated (?) | ||
| + | # pink team +template, good | ||
| + | # green team -template, good | ||
| + | # green team +template, good | ||
| + | # yellow team +template, good | ||
| + | # empty | ||
| + | |||
| + | ===Bottom row, Wed/Fri Section Samples=== | ||
Revision as of 14:10, 6 April 2007
On Day 1 of Module 3, you did a few things:
- Learned about the SAGA chromatin-remodeling complex and chose a non-essential sub-unit of the SAGA
complex to delet
- Designed primers with landing se
Gel lane loading order
Top row, Tue/Thur Section Samples
- empty
- ladder (100bp)
- purple team 1
- purple team 2
- blue team -template, good
- blue team +template, good
- red team +template, good
- red team -template, good
- pink team -template, contaminated (?)
- pink team +template, good
- green team -template, good
- green team +template, good
- yellow team +template, good
- empty
