Talk:20.109(S07): Start-up expression engineering

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On Day 1 of Module 3, you did a few things:
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# Learned about the SAGA chromatin-remodeling complex and chose a non-essential sub-unit of the SAGA
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  complex to delet
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# Designed primers with landing se
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[[Image:Gel mod3day1 PCR products.JPG]]
[[Image:Gel mod3day1 PCR products.JPG]]
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==Gel lane loading order==
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===Top row, Tue/Thur Section Samples===
# empty
# empty
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# red team +template, good
# red team +template, good
# red team -template, good
# red team -template, good
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# pink team -template, contaminated (?)
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# pink team +template, good
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# green team -template, good
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# green team +template, good
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# yellow team +template, good
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# empty
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===Bottom row, Wed/Fri Section Samples===

Revision as of 14:10, 6 April 2007

On Day 1 of Module 3, you did a few things:

  1. Learned about the SAGA chromatin-remodeling complex and chose a non-essential sub-unit of the SAGA
 complex to delet
  1. Designed primers with landing se

Image:Gel mod3day1 PCR products.JPG

Gel lane loading order

Top row, Tue/Thur Section Samples

  1. empty
  2. ladder (100bp)
  3. purple team 1
  4. purple team 2
  5. blue team -template, good
  6. blue team +template, good
  7. red team +template, good
  8. red team -template, good
  9. pink team -template, contaminated (?)
  10. pink team +template, good
  11. green team -template, good
  12. green team +template, good
  13. yellow team +template, good
  14. empty

Bottom row, Wed/Fri Section Samples

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