Talk:20.109(S08):Testing cell viability (Day3)

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Revision as of 16:55, 25 April 2008

Please write your team colour next to the spot that you would like.

Feel free to arrive earlier than your scheduled time!

Contents

T/R schedule

Microscope time Arrival time (at latest!) Team colour
2:30-2:55 1:05 Yellow
2:55-3:20 1:25 Pink
3:20-3:45 1:50 Red
3:45-4:10 2:15 Purple
4:10-4:35 2:40 Green
4:35-5:00 3:05 Blue

W/F schedule

Microscope time Arrival time (at latest!) Team colour
2:30-2:55 1:05 Purple
2:55-3:20 1:25 Red
3:20-3:45 1:50 Blue
3:45-4:10 2:15 Green
4:10-4:35 2:40 Pink
4:35-5:00 3:05 Yellow

T/R pictures

Please post one picture for each of your two alginate samples (you can post just the green channel).

Caption for Agi's example pictures, 40X, used 1% alginate
Caption for Agi's example pictures, 40X, used 1% alginate
Figure: Yellow Group. 10X FITC filter. 3D 1% alginate chondrocyte cells with no collagen II.
Figure: Yellow Group. 10X FITC filter. 3D 1% alginate chondrocyte cells with no collagen II.
Figure: Pink Group. 10X FITC filter. 3D 1% alginate chondrocyte cells with no toxin added.
Figure: Pink Group. 10X FITC filter. 3D 1% alginate chondrocyte cells with no toxin added.
Figure: Pink Group. 10X FITC filter. 3D 1% alginate chondrocyte cells with toxin added.
Figure: Pink Group. 10X FITC filter. 3D 1% alginate chondrocyte cells with toxin added.
Figure: Pink Group. 10X FITC filter. 2D chondrocyte cells with rich media.
Figure: Pink Group. 10X FITC filter. 2D chondrocyte cells with rich media.
Figure: Red Group. 10X FITC filter. 2D chondrocyte cells with media w/o ascorbate.
Figure: Red Group. 10X FITC filter. 2D chondrocyte cells with media w/o ascorbate.
Figure: Red Group. 10X FITC filter. 3D 2% Sigma Aldrich alginate in rich media.
Figure: Red Group. 10X FITC filter. 3D 2% Sigma Aldrich alginate in rich media.
Figure: Purple Group. 10X FITC filter. 2D sample.
Figure: Purple Group. 10X FITC filter. 2D sample.
Figure: Green Group. 10X FITC filter. 2D sample.
Figure: Green Group. 10X FITC filter. 2D sample.
Figure: Green Group. 10X FITC filter. 3D sample. 5million in 1%alginate
Figure: Green Group. 10X FITC filter. 3D sample. 5million in 1%alginate
Figure: Green Group. 10X FITC filter. 2D sample. 20million in 1%alginate
Figure: Green Group. 10X FITC filter. 2D sample. 20million in 1%alginate
Figure: Blue Group. 10X FITC filter. 2D sample. 1million cells
Figure: Blue Group. 10X FITC filter. 2D sample. 1million cells


W/F pictures

Please post one picture for each of your two alginate samples (you can post just the green channel).

Caption for Agi's example pictures, 40X, used 1% alginate
Caption for Agi's example pictures, 40X, used 1% alginate
Purple Group. Left Figure: 10X FITC Filter, 2D Sample. Right Figure: 10X EthD-1 Filter, 3D Sample in 1% alginate
Purple Group. Left Figure: 10X FITC Filter, 2D Sample. Right Figure: 10X EthD-1 Filter, 3D Sample in 1% alginate
Red Group. Left figure: 10X FITC filter, 3D with mechanical stress, 1% alginate.  Right figure: 10X FITC Filter, 2D 1% alginate
Red Group. Left figure: 10X FITC filter, 3D with mechanical stress, 1% alginate. Right figure: 10X FITC Filter, 2D 1% alginate
Blue Group. 10X FITC filter, 3D normal calcium concentration, 2% alginate
Blue Group. 10X FITC filter, 3D normal calcium concentration, 2% alginate
Blue Group. 10X EthD-1 Filter, 3D normal calcium concentration, 2% alginate
Blue Group. 10X EthD-1 Filter, 3D normal calcium concentration, 2% alginate
Green Group. 10X FITC filter, 2D normal culture.
Green Group. 10X FITC filter, 2D normal culture.
Green Group. 10X FITC filter, 3D 2% Sigma brand low viscosity alginate culture.
Green Group. 10X FITC filter, 3D 2% Sigma brand low viscosity alginate culture.
Pink Group. 10X FITC filter, control culture in 1% alginate.
Pink Group. 10X FITC filter, control culture in 1% alginate.
Pink Group. 10X FITC filter, weighted culture in 1% alginate. Weights were used to test response of chondrocyes to mechanical stress.
Pink Group. 10X FITC filter, weighted culture in 1% alginate. Weights were used to test response of chondrocyes to mechanical stress.
Yellow Group. 2D sample 10X
Yellow Group. 2D sample 10X
Yellow Group. 2D sample 40X
Yellow Group. 2D sample 40X
Yellow Group. 3D sample 10X
Yellow Group. 3D sample 10X



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