Talk:20.109(S09):Data analysis (Day8)

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(W/F data)
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|S101L and E127K data was noisy
|S101L and E127K data was noisy
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|Kd=0.8355, n=2.5073
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|Kd=0.5218, n=7.5210
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|Kd=0.4948, n=7.6035
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|T79P seems to have increased cooperativity
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Revision as of 17:42, 6 March 2009

T/R data

Below is your calcium titration data, as well as data from pilot experiments done by the teaching faculty for comparison.

Please fill in the second table by Sunday, if not today, so your classmates can compare their data to yours.


Day 7 data file Data taken after 24 hr settling period
Media:S09_M1D7_TeachingExample.xls Mutations: WT, S101L, Y64D
Media:S09_M1D7_TR_Blue.txt
Media:S09_M1D7_TR_Green.txt Media:S09_M1D7_TR_Green-redo.txt
Media:S09_M1D7_TR_Grey.txt Media:S09_M1D7_TR_Grey-redo.txt
Media:S09_M1D7_TR_Orange.txt
Media:S09_M1D7_TR_Pink.txt Media:S09_M1D7_TR_Pink-redo.txt
Media:S09_M1D7_TR_Purple.txt Media:S09_M1D7_TR_Purple-redo.txt
Media:S09_M1D7_TR_Red.txt
Media:S09_M1D7_TR_Yellow.txt


Group Colour X#Z Mutant Most believable KD; n for X#Z mutant Most believable KD; n for S101L Most believable KD; n for WT Comments
Teaching Faculty Y64D N/A ~ 0.5 μM ; ~ 8 ~ 0.3 μM; ~ 1(.2) Y64D killed calcium response
Yellow Y64D N/A ~0.4 μM; ~7 ~0.5 μM; ~3 No Calcium binding in Y64D

W/F data

Below is your calcium titration data, as well as data from pilot experiments done by the teaching faculty for comparison.

Please fill in the second table by Sunday, if not today, so your classmates can compare their data to yours.


Day 7 data file Data taken after 24 hr settling period
Media:S09_M1D7_TeachingExample.xls Mutations: WT, S101L, Y64D
Media:S09_M1D7_WF_Blue.txt
Media:S09_M1D7_WF_Green.txt
Media:S09_M1D7_WF_Grey.txt
Media:S09_M1D7_WF_Orange-1.txt
Media:S09_M1D7_WF_Orange-2.txt
Media:S09_M1D7_WF_Orange-redo.txt
Media:S09_M1D7_WF_Pink.txt
Media:S09_M1D7_WF_Purple-1.txt
Media:S09_M1D7_WF_Purple-2.txt
Media:S09_M1D7_WF_Red.txt
Media:S09_M1D7_WF_Yellow.txt Media:S09_M1D7_WF_Yellow-redo.txt


Group Colour X#Z Mutant Most believable KD; n for X#Z mutant Most believable KD; n for S101L Most believable KD; n for WT Comments
Teaching Faculty Y64D N/A ~ 0.5 μM ; ~ 8 ~ 0.3 μM ; ~ 1(.2) Y64D killed calcium response
Orange E127K Kd-.4558, n-1.2418 N/A (using teaching data) Kd=.6285, n=.1490 S101L and E127K data was noisy
Green T79P Kd=0.8355, n=2.5073 Kd=0.5218, n=7.5210 Kd=0.4948, n=7.6035 T79P seems to have increased cooperativity
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