Talk:20.109(S12):Assess protein function (Day7)

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(Analysis, W/F)
(Analysis, T/R)
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|0.166, 1.30
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|I utilized model 2.
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Revision as of 14:59, 18 April 2012

Raw Data

Please post your raw calcium titration data below.


Day 7 data file, T/R Day 7 data file, W/F
Media:S12_M2D7_TR-Red.txt Media:S12_M2D7_WF-Red.txt
Media:S12_M2D7_TR-Orange.txt Media:S12_M2D7_WF-Orange.txt
Media:S12_M2D7_TR-Yellow.txt Media:S12_M2D7_WF-Yellow.txt
Media:S12_M2D7_TR-Green.txt Media:S12_M2D7_WF-Green.txt
Media:S12_M2D7_TR-Blue.txt Media:S12_M2D7_WF-Blue.txt
Media:S12_M2D7_TR-Pink.txt Media:S12_M2D7_WF-Pink.txt
Media:S12_M2D7_TR-Purple.txt Media:S12_M2D7_WF-Purple.txt


Analysis, T/R

Please be mindful of significant figures when you fill in this table. Do not state far more precise numbers than you can believe.

Group Colour E67K, T79P, or M124S? X#Z Mutant Most believable KD; n for WT Most believable KD; n for (+) Most believable KD; n for X#Z mutant Comments
Orange T79P D24H 0.104, 1.26 0.166, 1.30 0.7892, 2.73 I utilized model 2.

Analysis, W/F

Please be mindful of significant figures when you fill in this table. Do not state far more precise numbers than you can believe.

Group Colour E67K, T79P, or M124S? X#Z Mutant Most believable KD; n for WT Most believable KD; n for (+) Most believable KD; n for X#Z mutant Comments
Orange T79P V55F KD 0.1025; n 1.8227 for WT KD 0.1491; n 1.3982 for T79P KD 0.1598; n 1.6114 for V55F The WT and T79P were switched during the experiment. For the above results, the labels were switched back to the original
Red T79P T79F KD 0.1075; n 1.3550 for WT KD 0.1372; n 1.1433 for T79P KD 0.1053; n 1.3550 for T79F As the above group, the labels were switched back to the original between T79P and WT. Also, our yield for WT and T79P was low (may be error prone)
Pink E67K D20K KD 0.1628; n 0.8206 for WT KD 0.0520; n 0.2212 for E67K KD 0.000047 for D20K we used KDs from model 1
Purple M124S G23W/G25W KD 0.1643; n 1.3591 for WT KD 0.4753; n 2.9004 for M124S KD 0.1470; n 1.0503 for G23W/G25W Our yields for WT and G23W/G25W were low. The fluorescence values for G23W/G25W were very low (only between 18 and 24) and may not be significant. For G23W/G25W (contrary to WT and M124S), as [Ca2+] increased, fluorescence increased.
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