Talk:20.109(S13):Assess protein function (Day8)

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(Analysis, T/R)
(Analysis, T/R)
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==Analysis, W/F==
==Analysis, W/F==
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==Analysis, T/R==
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==Analysis, W/F==
Please be mindful of significant figures when you fill in this table. Do not state far more precise numbers than you can believe.
Please be mindful of significant figures when you fill in this table. Do not state far more precise numbers than you can believe.

Revision as of 15:15, 12 April 2013

Contents

Raw Data

Raw calcium titration data is posted below.


Day 7 data file, T/R Day 7 data file, W/F
Media:S13-M2D7-TR-Red.txt Media:S13-M2D7-WF-Red.txt
Media:S13-M2D7-TR-Org.txt Media:S13-M2D7-WF-Org.txt
Media:S13-M2D7-TR-Ylw.txt Media:S13-M2D7-WF-Ylw.txt
Media:S13-M2D7-TR-Grn.txt Media:S13-M2D7-WF-Grn.txt
Media:S13-M2D7-TR-Blu.txt Media:S13-M2D7-WF-Blu.txt
N/A Media:S13-M2D7-WF-Pnk.txt
Media:S13-M2D7-TR-Prp.txt Media:S13-M2D7-WF-Prp.txt
Media:S13-M2D7-TR-Plt.txt Media:S13-M2D7-WF-Plt.txt


Analysis, T/R

Please be mindful of significant figures when you fill in this table. Do not state far more precise numbers than you can believe.

Group Colour D24H, E67K, T79P, or M124S? X#Z Mutant Most believable KD; n for WT Most believable KD; n for reference Most believable KD; n for X#Z mutant Comments (e.g., very low fluorescence)
Plt M124S D80R WT: 0.0976; 1.4751 M124S: 0.4115; 3.9341 D80R: 1.2991; 2.8465 small difference between 0% and 100% for our mutant, affinity decrease and cooperativity increase
Orange D24H G25Q 0.1462, n = 0.8310 0.6759, n = 2.5734 0.7118; n = -0.2595 Very low fluorescence; oscillating trend...
Purple M124S M144R KD = 0.0672, n = 1.1286 KD = 0.2682, n= 3.3655 KD = 0.3583, n= 4.1618 Higher cooperativity, lower affinity in target mutant
Blue M124S M71R KD = 0.1175, n = 1.4880 KD = 0.3377, n = 3.3863 KD = 0.2246, n = 0.6741 Negative cooperativity, lower affinity in target mutant

Analysis, W/F

Analysis, W/F

Please be mindful of significant figures when you fill in this table. Do not state far more precise numbers than you can believe.

Group Colour D24H, E67K, T79P, or M124S? X#Z Mutant Most believable KD; n for WT Most believable KD; n for reference Most believable KD; n for X#Z mutant Comments (e.g., very low fluorescence)
Blue D24H D22E Kd= 0.0778, n= 1.2080 Kd= 0.6353, n= 3.7325 Kd= 0.6892, n= 7.8230 Affinity decreased while the hill coefficient increased significantly. No extreme outliers.
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