Talk:20.109(S13):Characterize protein expression (Day7): Difference between revisions
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[[Image:S13_M2D7_WF_Green.JPG | right |thumb|250px | W/F Gel, Green group -- OK]] | [[Image:S13_M2D7_WF_Green.JPG | right |thumb|250px | W/F Gel, Green group -- OK]] | ||
[[Image:S13_M2D7_WF_Blue.JPG | left |thumb|250px | W/F Gel, Blue group -- OK]] | [[Image:S13_M2D7_WF_Blue.JPG | left |thumb|250px | W/F Gel, Blue group -- OK]] | ||
[[Image:S13_M2D7_WF_Pink.JPG | center |thumb|250px | W/F Gel, Pink group]] | [[Image:S13_M2D7_WF_Pink.JPG | center |thumb|250px | W/F Gel, Pink group -- really T/R Blue!]] | ||
[[Image:S13_M2D7_WF_Purple.JPG | left |thumb|250px | W/F Gel, Purple group -- really Pink]] | [[Image:S13_M2D7_WF_Purple.JPG | left |thumb|250px | W/F Gel, Purple group -- really Pink]] | ||
[[Image:S13_M2D7_WF_Platinum.JPG | center |thumb|250px | W/F Gel, Platinum group -- really Purple]] | [[Image:S13_M2D7_WF_Platinum.JPG | center |thumb|250px | W/F Gel, Platinum group -- really Purple]] | ||
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Latest revision as of 14:32, 18 April 2013
SDS-PAGE analysis tips
You can look up the expected molecular weight of IPC using the sequence file (from Day 1 or Day 5) and this link or a similar website. Be sure to add ~ 3 KDa for the size of the N-terminus of pRSET (His tag, etc)!
If you see two strong bands on your gel, what do you think the second one is? As a starting hint, look back at the Day 6 protocol and carefully consider what is present in the unpurified protein tubes.
What other assays might you consider in relation to SDS-PAGE in order to take a holistic view of your data?
SDS-PAGE images
T/R
W/F
