Talk:20.109(S13):Initiate cell culture (Day2)

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==Designs (T/R)==
==Designs (T/R)==
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Here, please!
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Purple: Our plan is to culture chondrocytes under standard system conditions, but add an insulin supplement to the media at two concentrations higher than that already present in the ITS/FCS media. Insulin has been found to inhibit dedifferentiation of chondrocytes to the fibroblast state. We think our first 3D sample, subjected to 10 ug/day of insulin will inhibit dedifferentiation less than our second 3D sample, subjected to 20 ug/day, because a higher concentration of insulin will result in a larger inhibitory effect in the cell culture.
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Platinum: Our plan is to culture stem cells under standard low viscosity alginate conditions but add growth factor BMP2 to the media. BMP2 has been found to induce chondrogenesis at certain concentrations. We think our first 3D sample, a negative control not subjected to BMP2 will show less of a chondrocyte phenotype than our second 3D sample, subjected to ___ ug/ mL BMP2 / day.
==Designs (W/F)==
==Designs (W/F)==
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Here, please!
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Orange: We propose to study the effect of varying initial cell densities to determine an optimal starting density for the maintenance of chondrocyte differentiation. We hypothesize that lower cell density will result in a slower rate of central viability loss from due to the fact that in high density, cells can signal those around them and potentially disrupt central viability.

Revision as of 23:03, 22 April 2013

Contents

Plan for Day 2

Group 1 should arrive by 1:05 pm at the latest and immediately go to the tissue culture room. When everyone has arrived, we will thaw your cells in the water bath. After you have finished your culture preparations (ideally by 3 pm), you can take a 10 minute break to refresh your minds, and then will take a short quiz.

Group 2 should arrive by 2:45 pm at the latest and will begin by taking the quiz. If all goes well, you will begin working in the tissue culture room at 3 pm. If your culture preparations that involve a special physical set-up, you can come earlier and work in the extra tissue culture hood.

Note that you may be asked to switch your group number based on grouping together people working with the same type of cells, asking people who need special reagents/equipment to go in the second group, etc.

T/R

Arrival time (at latest!) 1:05 pm 2:45 pm
Team colour 1 Blue Yellow
Team colour 2 Red Green
Team colour 3 Orange Purple
Team colour 4 Platinum

W/F

Arrival time (at latest!) 1:05 pm 2:45 pm
Team colour 1 Pink Red
Team colour 2 Green Blue
Team colour 3 Orange Purple
Team colour 4 Yellow

Designs (T/R)

Purple: Our plan is to culture chondrocytes under standard system conditions, but add an insulin supplement to the media at two concentrations higher than that already present in the ITS/FCS media. Insulin has been found to inhibit dedifferentiation of chondrocytes to the fibroblast state. We think our first 3D sample, subjected to 10 ug/day of insulin will inhibit dedifferentiation less than our second 3D sample, subjected to 20 ug/day, because a higher concentration of insulin will result in a larger inhibitory effect in the cell culture.

Platinum: Our plan is to culture stem cells under standard low viscosity alginate conditions but add growth factor BMP2 to the media. BMP2 has been found to induce chondrogenesis at certain concentrations. We think our first 3D sample, a negative control not subjected to BMP2 will show less of a chondrocyte phenotype than our second 3D sample, subjected to ___ ug/ mL BMP2 / day.

Designs (W/F)

Orange: We propose to study the effect of varying initial cell densities to determine an optimal starting density for the maintenance of chondrocyte differentiation. We hypothesize that lower cell density will result in a slower rate of central viability loss from due to the fact that in high density, cells can signal those around them and potentially disrupt central viability.

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