Talk:20.109(S13):Transcript-level analysis (Day6)

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Contents

qPCR data

T/R

The Excel file below contains all of the T/R data. Two different CP values are given. The first is calculated when all samples are thresholded at the same level. The second is calculated when a separate threshold is made for each primer set. (T/R Purple, because your samples ran separately, there were not enough of them to make a per RNA threshold reasonable.)

Media:20109-S13_TR_qPCR.xlsx

W/F

The Excel file below contains all of the T/R data. Two different CP values are given. The first is calculated when all samples are thresholded at the same level. The second is calculated when a separate threshold is made for each primer set.

Media:20109-S13_WF_qPCR.xlsx

qPCR section-wide curves

T/R section, Raw amplification curves
T/R section, Raw amplification curves
T/R section, Log-scale amplification curves with noise threshold
T/R section, Log-scale amplification curves with noise threshold
T/R section, Melt curves for 18 samples
T/R section, Melt curves for 18 samples
T/R section, Melt curves for CN II samples
T/R section, Melt curves for CN II samples
T/R section, Melt curves for CN II samples
T/R section, Melt curves for CN II samples



W/F section, Raw amplification curves
W/F section, Raw amplification curves
W/F section, Log-scale amplification curves with noise threshold
W/F section, Log-scale amplification curves with noise threshold
W/F section, Melt curves for 18 samples
W/F section, Melt curves for 18 samples
W/F section, Melt curves for CN II samples
W/F section, Melt curves for CN II samples
W/F section, Melt curves for CN II samples
W/F section, Melt curves for CN II samples


Cross-group meetings

T/R Scheme

Red 1/Yellow 1: Marisol/Dominique

Red 2/Blue 1: Nick/Justin

Orange 1/Platinum 2: Daphne/Katie

Platinum 1/Blue 2: Emily/Noa

Yellow 2/Green 2: Ebaa/Jesse

Green 1/Purple 1:Nikki/Sarah

Orange 2/Purple 2: Stas/Mitali

W/F Scheme

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