# Talk:20.109(S13):Transcript-level analysis (Day6)

(Difference between revisions)
 Revision as of 12:01, 3 May 2013 (view source) (→qPCR section-wide curves)← Previous diff Revision as of 15:22, 6 May 2013 (view source) (→qPCR data)Next diff → Line 3: Line 3: ===T/R=== ===T/R=== - The text file below contains all of the T/R data. Two different CP values are given. The first is calculated when all samples are thresholded at the same level. The second is calculated when a separate threshold is made for each primer set. (T/R Purple, because your samples ran separately, there were not enough of them to make a per RNA threshold reasonable.) + The Excel file below contains all of the T/R data. Two different CP values are given. The first is calculated when all samples are thresholded at the same level. The second is calculated when a separate threshold is made for each primer set. (T/R Purple, because your samples ran separately, there were not enough of them to make a per RNA threshold reasonable.) - [[Media:20109-S13_TR_qPCR.txt]] + [[Media:20109-S13_TR_qPCR.xlsx]] ===W/F=== ===W/F=== - The text file below contains all of the T/R data. Two different CP values are given. The first is calculated when all samples are thresholded at the same level. The second is calculated when a separate threshold is made for each primer set. + The Excel file below contains all of the T/R data. Two different CP values are given. The first is calculated when all samples are thresholded at the same level. The second is calculated when a separate threshold is made for each primer set. - [[Media:20109-S13_WF_qPCR.txt]] + [[Media:20109-S13_WF_qPCR.xlsx]] ==qPCR section-wide curves== ==qPCR section-wide curves==

## qPCR data

### T/R

The Excel file below contains all of the T/R data. Two different CP values are given. The first is calculated when all samples are thresholded at the same level. The second is calculated when a separate threshold is made for each primer set. (T/R Purple, because your samples ran separately, there were not enough of them to make a per RNA threshold reasonable.)

### W/F

The Excel file below contains all of the T/R data. Two different CP values are given. The first is calculated when all samples are thresholded at the same level. The second is calculated when a separate threshold is made for each primer set.

## qPCR section-wide curves

T/R section, Raw amplification curves
T/R section, Log-scale amplification curves with noise threshold
T/R section, Melt curves for 18 samples
T/R section, Melt curves for CN II samples
T/R section, Melt curves for CN II samples

W/F section, Raw amplification curves
W/F section, Log-scale amplification curves with noise threshold
W/F section, Melt curves for 18 samples
W/F section, Melt curves for CN II samples
W/F section, Melt curves for CN II samples

## Cross-group meetings

### T/R Scheme

Red 1/Yellow 1: Marisol/Dominique

Red 2/Blue 1: Nick/Justin

Orange 1/Platinum 2: Daphne/Katie

Platinum 1/Blue 2: Emily/Noa

Yellow 2/Green 2: Ebaa/Jesse

Green 1/Purple 1:Nikki/Sarah

Orange 2/Purple 2: Stas/Mitali