Talk:20.109(S13): Spring 2013 schedule

TEMP AGI

MODULE	DAY	DATE	LAB
N/A 0		Tue/Wed Feb 5/6	Orientation
1	1	Thur/Fri Feb 7/8	Context-setting and primer design
1	2	Tue/Wed Feb 12/13	DNA extraction
1	3	Thur/Fri Feb 14/15	PCR and paper discussion
President's Day Feb 18			No lab Feb 19 or Feb 20
1	4	Thur/Fri Feb 21/22	DNA cloning
1	5	Tue/Wed Feb 26/27	DNA sequencing and primer analysis
1	6	Thur/Fri Feb 28/ Mar 1	Journal club I
1	7	Tue/Wed Mar 5/6	Phylogenetic analysis
1	8	Thur/Fri Mar 7/8, No lecture today	Journal club II
2	1	Tue/Wed Mar 12/13	Design mutant Module 1 Draft Due
2	2	Thur/Fri Mar 14/15	Site-directed mutagenesis
2	3	Tue/Wed Mar 19/20	Bacterial amplification of DNA *Module 1 Primer Summary Due
2	4	Thur/Fri Mar 21/22	Prepare expression system
Spring Break Mar 25-29			No lab all week
2	5	Tue/Wed Apr 2/3	Induce protein and evaluate DNA
2	6	Thur/Fri Apr 4/5	Purify protein Module 1 Revision Due
2	7	Tue/Wed Apr 9/10	Characterize protein expression
2	8	Thur/Fri Apr 11/12	Assess protein function
Patriot's Day April 15-16			No lab Apr 16 or Apr 17
3	1	Thur/Fri Apr 18/19	Design experiment
3	2	Tue/Wed Apr 23/24	Initiate cell culture Module 2 Report Due MONDAY APRIL 22 by 5 pm for all.
3	3	Thur/Fri Apr 25/26	Testing cell viability
3	4	Tue/Wed Apr 30/May 1	Preparing cells for analysis
3	5	Thur/Fri May 2/3	Initiating transcript and protein assays
3	6	Tue/Wed May 7/8	Transcript-level analysis
3	7	Thur/Fri May 9/10	Protein-level and wrap-up analysis Module 3 Summary Due
3	8	Tue/Wed May 14/15	Student presentations