Talk:20.109(S14):Diagnostic primer design (Day2)

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Sensitivity)
(Sensitivity)
Line 100: Line 100:
|CCA GGT TGA CTC TGC CTC ACG TCG
|CCA GGT TGA CTC TGC CTC ACG TCG
|  
|  
 +
| BP 2/3
|  
|  
-
|  
+
| VC/EH SSU rRNA
-
|
+
|-
|-
|Yellow
|Yellow

Revision as of 14:44, 13 February 2014

Contents

T/R Section

Sensitivity

Team color Forward (F) primer Reverse (R) primer Target site (e.g. bp 60), F Target side (e.g., bp 300), R Is bp # for VC or EH? Which gene?
Red CAC CAG GTT GAT ACT GCC TGA C CCT ATC CCG AAC CAA ACC CTG bp 1 bp 260 EH, SSU rRNA
Green
Pink ACC AGG TTG ATT CTG CCT GAC TCT CTC ACG CTC CCT CTC C bp1/bp2 bp252/bp290 EH/VC SSU rRna


Specificity

Team Color Forward primer Reverse primer Target site (e.g. bp 60), F Target side (e.g., bp 300), R Is bp # for VC or EH? Which gene?
Yellow CCAGGTTGATTCTGCCTGACG CCGGAGAGGGAGCCTGAG bp 2 (VC) / bp 3 (EH) bp 250 (VC) / bp 288 (EH) VC/EH, SSU rRNA
Blue GATTCTGCCTGACGTAGATGC CTGCTGGCACCAAACTTGC 10 bp 408 bp E. hellem (AF118143)
Orange


W/F Section

Sensitivity

Team color Forward (F) primer Reverse (R) primer Target site (e.g. bp 60), F Target site (e.g., bp 300), R Is bp # for VC or EH? Which gene?
Pink ACC AGG TTG ATT CTG CTG ACG TTGCGAGCATACTATCCCCAG bp 1/2 bp 741/768 VC/EH SSU rRNA
Red ACC AGG TTG ATT CTG CCT GAC ATA TTA CCG CGG CTG CTG bp 1/2 bp 380/421 VC/EH small subunit rRNA
Orange CCA GGT TGA CTC TGC CTC ACG TCG BP 2/3 VC/EH SSU rRNA
Yellow CAC CAG GTT GAT TCT GCC TGA C CCA TCC ATT TCC AAC GAC CAT G 1 947 EH


Specificity

Team color Forward (F) primer Reverse (R) primer Target site (e.g. bp 60), F Target site (e.g., bp 300), R Is bp # for VC or EH? Which gene?
White
Blue CAC CAG GTT GAT TCT GCC TGA C CTG CTG GCA CCA GAC TTG C 1 426 for EH; 386 for VC Both. SSU rRNA
Silver CAC CAG GTT GAT TCT GCC TGA C TTC ACC ATA GAC ACT ACT GGA GC 1 441 EH
Purple GTCTGGCTGTGTAGATAACTACG CACTGTGAGACCTCGCATC 102 465 VC
Green agattgctcccacgtccaag caacgaccatgcaccactc 251 872 VC



Personal tools