Talk:20.109(S14):Transcript-level analysis (Day6)

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(qPCR section-wide curves)
(Files with CP values and additional notes)
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[[Media:20109-S14_TR_qPCR.xlsx]]<br>
[[Media:20109-S14_TR_qPCR.xlsx]]<br>
[[Media:20109-S14_WF_qPCR.xlsx]]
[[Media:20109-S14_WF_qPCR.xlsx]]
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Note that the WF file contains two spreadsheets, one for the original in-class run, and one for the additional run for Yellow (A) and Green (B) groups. '''Note that everyone can learn something about cross-plate replication''' from Yellow team's data (C in original run) if they wish to.
==qPCR section-wide curves==
==qPCR section-wide curves==

Revision as of 15:01, 6 May 2014

Contents

T/R pairings


W/F pairings


qPCR data

Files with CP values and additional notes

The Excel files below contains all of the section-wide data. Two different CP values are listed per sample. The first is calculated when all samples are thresholded at the same level. The second is calculated when a separate threshold is made for each primer set.

Media:20109-S14_TR_qPCR.xlsx
Media:20109-S14_WF_qPCR.xlsx

Note that the WF file contains two spreadsheets, one for the original in-class run, and one for the additional run for Yellow (A) and Green (B) groups. Note that everyone can learn something about cross-plate replication from Yellow team's data (C in original run) if they wish to.

qPCR section-wide curves

T/R

T/R section, Raw amplification curves
T/R section, Raw amplification curves
T/R section, Log-scale amplification curves with noise threshold
T/R section, Log-scale amplification curves with noise threshold
T/R section, Melt curves for 18S samples
T/R section, Melt curves for 18S samples
T/R section, Melt curves for CN II samples
T/R section, Melt curves for CN II samples
T/R section, Melt curves for CN I samples
T/R section, Melt curves for CN I samples
T/R section, Amp curves for 18S samples
T/R section, Amp curves for 18S samples
T/R section, Amp curves for CN II samples
T/R section, Amp curves for CN II samples
T/R section, Amp curves for CN I samples
T/R section, Amp curves for CN I samples


W/F

Original run

W/F section, Raw amplification curves
W/F section, Raw amplification curves
W/F section, Log-scale amplification curves with noise threshold
W/F section, Log-scale amplification curves with noise threshold
W/F section, Melt curves for 18 samples
W/F section, Melt curves for 18 samples
W/F section, Melt curves for CN II samples
W/F section, Melt curves for CN II samples
W/F section, Melt curves for CN I samples
W/F section, Melt curves for CN I samples
W/F section, Amp curves for 18S samples
W/F section, Amp curves for 18S samples
W/F section, Amp curves for CN II samples
W/F section, Amp curves for CN II samples
W/F section, Amp curves for CN I samples
W/F section, Amp curves for CN I samples


Extra run for Ylw and Grn groups

W/F section, Raw amplification curves
W/F section, Raw amplification curves
W/F section, Log-scale amplification curves with noise threshold
W/F section, Log-scale amplification curves with noise threshold
W/F section, Melt curves for 18 samples
W/F section, Melt curves for 18 samples
W/F section, Melt curves for CN II samples
W/F section, Melt curves for CN II samples
W/F section, Melt curves for CN I samples
W/F section, Melt curves for CN I samples
W/F section, Amp curves for 18S samples
W/F section, Amp curves for 18S samples
W/F section, Amp curves for CN II samples
W/F section, Amp curves for CN II samples
W/F section, Amp curves for CN I samples
W/F section, Amp curves for CN I samples
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