Talk:BE.109: Difference between revisions

Revision as of 14:06, 5 May 2006

RS 17:06, 5 May 2006 (EDT): Make a version of OpenWetWare:Getting started specifically geared towards BE.109. (Reshma should do this.)

Revision as of 14:17, 3 April 2006 (view source) Mhfoley (talk \| contribs) (Possible improvements in the Protein Engineering module for next year)		Revision as of 14:06, 5 May 2006 (view source) Reshma P. Shetty (talk \| contribs) No edit summary Newer edit →
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	~~Suggestion 1~~: ~~On day 2 we ran gels of the cell lysates from induced and uninduced e coli. It might be~~ a ~~good idea to load a sample~~ of ~~recombinant beta-gal on these gels to make it more clear where the induced beta-gal might be running on the gel.~~		*'''[[User:Rshetty\|RS]] 17:06, 5 May 2006 (EDT)''': Make a version of [[OpenWetWare:Getting started]] specifically geared towards [[BE.109]]. (Reshma should do this.)

	~~Suggestion 2~~: ~~On day 3 we purified beta-gal from our cell lysates and measured activity of the purified material~~. ~~It might be fun for the students to measure activity of the supernatant as well~~. ~~This would give them the opportunity to think about the efficiency of their purification and some steps that they might take to improve efficiency if it turns out their recovery was less than optimal~~.