Talk:BE.109 - Revision history

Nkuldell at 10:40, 7 May 2006

Nkuldell — Sun, 07 May 2006 10:40:24 GMT

←Older revision		Revision as of 10:40, 7 May 2006
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	'''[[User:Yeem\|yeem]] 21:59, 6 May 2006 (EDT)'''' Made a couple changes. Didn't know what to do for "posting your data"; should we be encouraging people to post on their user pages?		'''[[User:Yeem\|yeem]] 21:59, 6 May 2006 (EDT)'''' Made a couple changes. Didn't know what to do for "posting your data"; should we be encouraging people to post on their user pages?
		+
		+	*'''[[User:Nkuldell\|Natalie]]''' I would prefer to have data posted on the discussion page associated with the day in lab, believing it will keep the information better organized for the class overall as well as allowing folks to better compare their data to the data that others collect. Happy to hear other thoughts though. I also wanted to thank you both for working on this now.

Yeem: couple changes

Yeem — Sun, 07 May 2006 01:59:33 GMT

couple changes

←Older revision		Revision as of 01:59, 7 May 2006
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	*'''[[User:Rshetty\|RS]] 10:51, 6 May 2006 (EDT)''': Starting the ball rolling. [[BE.109:Getting started on OpenWetWare]]		*'''[[User:Rshetty\|RS]] 10:51, 6 May 2006 (EDT)''': Starting the ball rolling. [[BE.109:Getting started on OpenWetWare]]
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		+	'''[[User:Yeem\|yeem]] 21:59, 6 May 2006 (EDT)'''' Made a couple changes. Didn't know what to do for "posting your data"; should we be encouraging people to post on their user pages?

Reshma P. Shetty at 14:51, 6 May 2006

Reshma P. Shetty — Sat, 06 May 2006 14:51:15 GMT

←Older revision		Revision as of 14:51, 6 May 2006
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	**'''[[User:Yeem\|yeem]] 17:25, 5 May 2006 (EDT)''': I'd be willing to help.		**'''[[User:Yeem\|yeem]] 17:25, 5 May 2006 (EDT)''': I'd be willing to help.
	**'''[[User:Rshetty\|RS]] 17:46, 5 May 2006 (EDT)''': Cool. Will be in touch.		**'''[[User:Rshetty\|RS]] 17:46, 5 May 2006 (EDT)''': Cool. Will be in touch.
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		+	*'''[[User:Rshetty\|RS]] 10:51, 6 May 2006 (EDT)''': Starting the ball rolling. [[BE.109:Getting started on OpenWetWare]]

Reshma P. Shetty at 21:46, 5 May 2006

Reshma P. Shetty — Fri, 05 May 2006 21:46:22 GMT

←Older revision		Revision as of 21:46, 5 May 2006
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	*'''[[User:Rshetty\|RS]] 17:06, 5 May 2006 (EDT)''': Make a version of [[OpenWetWare:Getting started]] specifically geared towards [[BE.109]]. (Reshma should do this.)		*'''[[User:Rshetty\|RS]] 17:06, 5 May 2006 (EDT)''': Make a version of [[OpenWetWare:Getting started]] specifically geared towards [[BE.109]]. (Reshma should do this.)
-	**I'd be willing to help. [[User:~~Yeem~~\|~~yeem~~]] 17:25, 5 May 2006 (EDT)	+	**'''[[User:Yeem\|yeem]] 17:25, 5 May 2006 (EDT)''': I'd be willing to help.
		+	**'''[[User:Rshetty\|RS]] 17:46, 5 May 2006 (EDT)''': Cool. Will be in touch.

Yeem: willing to help

Yeem — Fri, 05 May 2006 21:25:16 GMT

willing to help

←Older revision		Revision as of 21:25, 5 May 2006
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	*'''[[User:Rshetty\|RS]] 17:06, 5 May 2006 (EDT)''': Make a version of [[OpenWetWare:Getting started]] specifically geared towards [[BE.109]]. (Reshma should do this.)		*'''[[User:Rshetty\|RS]] 17:06, 5 May 2006 (EDT)''': Make a version of [[OpenWetWare:Getting started]] specifically geared towards [[BE.109]]. (Reshma should do this.)
		+	**I'd be willing to help. [[User:Yeem\|yeem]] 17:25, 5 May 2006 (EDT)

Reshma P. Shetty at 21:06, 5 May 2006

Reshma P. Shetty — Fri, 05 May 2006 21:06:30 GMT

←Older revision		Revision as of 21:06, 5 May 2006
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-	~~Suggestion 1~~: ~~On day 2 we ran gels of the cell lysates from induced and uninduced e coli. It might be~~ a ~~good idea to load a sample~~ of ~~recombinant beta-gal on these gels to make it more clear where the induced beta-gal might be running on the gel.~~	+	*'''[[User:Rshetty\|RS]] 17:06, 5 May 2006 (EDT)''': Make a version of [[OpenWetWare:Getting started]] specifically geared towards [[BE.109]]. (Reshma should do this.)
-		+
-	~~Suggestion 2~~: ~~On day 3 we purified beta-gal from our cell lysates and measured activity of the purified material~~. ~~It might be fun for the students to measure activity of the supernatant as well~~. ~~This would give them the opportunity to think about the efficiency of their purification and some steps that they might take to improve efficiency if it turns out their recovery was less than optimal~~.	+

Mhfoley: Possible improvements in the Protein Engineering module for next year

Mhfoley — Mon, 03 Apr 2006 21:17:12 GMT

Possible improvements in the Protein Engineering module for next year

New page

Suggestion 1: On day 2 we ran gels of the cell lysates from induced and uninduced e coli. It might be a good idea to load a sample of recombinant beta-gal on these gels to make it more clear where the induced beta-gal might be running on the gel.

Suggestion 2: On day 3 we purified beta-gal from our cell lysates and measured activity of the purified material. It might be fun for the students to measure activity of the supernatant as well. This would give them the opportunity to think about the efficiency of their purification and some steps that they might take to improve efficiency if it turns out their recovery was less than optimal.

←Older revision		Revision as of 14:51, 6 May 2006
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	**'''[[User:Yeem\|yeem]] 17:25, 5 May 2006 (EDT)''': I'd be willing to help.		**'''[[User:Yeem\|yeem]] 17:25, 5 May 2006 (EDT)''': I'd be willing to help.
	**'''[[User:Rshetty\|RS]] 17:46, 5 May 2006 (EDT)''': Cool. Will be in touch.		**'''[[User:Rshetty\|RS]] 17:46, 5 May 2006 (EDT)''': Cool. Will be in touch.
		+
		+	*'''[[User:Rshetty\|RS]] 10:51, 6 May 2006 (EDT)''': Starting the ball rolling. [[BE.109:Getting started on OpenWetWare]]

←Older revision		Revision as of 21:46, 5 May 2006
Line 1:		Line 1:
	*'''[[User:Rshetty\|RS]] 17:06, 5 May 2006 (EDT)''': Make a version of [[OpenWetWare:Getting started]] specifically geared towards [[BE.109]]. (Reshma should do this.)		*'''[[User:Rshetty\|RS]] 17:06, 5 May 2006 (EDT)''': Make a version of [[OpenWetWare:Getting started]] specifically geared towards [[BE.109]]. (Reshma should do this.)
-	**I'd be willing to help. [[User:~~Yeem~~\|~~yeem~~]] 17:25, 5 May 2006 (EDT)	+	**'''[[User:Yeem\|yeem]] 17:25, 5 May 2006 (EDT)''': I'd be willing to help.
		+	**'''[[User:Rshetty\|RS]] 17:46, 5 May 2006 (EDT)''': Cool. Will be in touch.