Talk:BE.109:Bio-material engineering/Sequence analysis
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Tuesday/Thursday Lab Sequence Data
team color | candidate number | sequence | relative strength | comments/notes |
Green | A | XXWAPAXXXXXX | 1/18 of pCT-CON | This sequence did not show significant binding ability, although because of the poor sequencing data, we can not make any definite conclusions on the cause of the poor gold binding ability. |
Green | B | XXXXVGXXXXXX | 3.5 of pCT-CON | This sequence has some gold binding ability, however unlike D, the sequence data obtained for this colony is quite poor. As a result, we can not make any definite conclusions on what lead to it's binding. |
Green | C | XXXXXXXXSLFL | .83 of pCT-CON | This sequence did not show significant binding ability, although because of the poor sequencing data, we can not make any definite conclusions on the cause of the poor gold binding ability. |
Green | D | LRXXTXGLSLCR | 7.83 of pCT-CON | Colony D was the only one from which we got some sort of meaningful sequence data from. Luckily, it was also our strongest binder. The sequence data shows a prevalence of Yellow Aliphatic Groups (Leucine) and Green Hydroxyl Groups (Serine), which may be important for gold binding. |
Purple | B | WRxxxACRCVxx | 0.3 x pCT-CON | 2 cysteines may be signficant |
Purple | C | MYYVAASxSVLM | 2 x pCT-CON | 2 methionines and 2 serines may be significant |
Purple | D | *LARPVA*xxIx | 10 x pCT-CON | best binder. stop codons are TAG and TGA |
Yellow | A | EXXXPIXXXXXX | 1.89 of pCT-CON | |
Yellow | B | RXRLRVXXXXVR | 0.413 of pCT-CON | |
Yellow | C | EGXXPXXXXXXX | 0.55 of pCT-CON | |
Yellow | D | FWCQSRWXEILV | 1.72 of pCT-CON | |
Pink | A | VAASRLVCCXXXL | 6 x pCT-CON | 2 cysteines right next to each other might mean that disulfide bonds cannot occur so sulfur available to bind to gold. |
Pink | D | XXADKLLXHAPQ* | 6 x pCT-CON | Histidine present which if we enrinched the species, histidine would probably be present in greater amounts :) |
Wednesday/Friday Lab Sequence Data
team color | candidate number | sequence | relative strength | comments/notes | |
HAVE A GREAT SUMMER!!