Talk:BE.109:Protein engineering/Purifying beta-galactosidase: Difference between revisions
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(T-Th Purple Team Data) |
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| Line 24: | Line 24: | ||
|- | |- | ||
| Purple | | Purple | ||
| | | Paramagnetic Beads | ||
| | | OD-595 = 0.5556(Conc.) - 0.0012 | ||
| | | 4.19 mg/mL | ||
| | | 0.0501 mg/mL | ||
| | | 37.37 units | ||
| | | 0.0506 mg/mL | ||
| | | 34.87 units | ||
|- | |- | ||
| Red | | Red | ||
Revision as of 03:45, 23 March 2006
To compare the purification protocols you have tried today, each team in each lab section should enter their data into the following tables.
Tuesday/Thursday
| Team Color | purification method | equation for BSA standard curve | concentration of beta-gal stock | concentration of purified beta-gal (wild type) | activity of purified beta-gal (wild type) | concentration of modified beta-gal (list modification below table) | activity of modified beta-gal |
|---|---|---|---|---|---|---|---|
| Green | |||||||
| Purple | Paramagnetic Beads | OD-595 = 0.5556(Conc.) - 0.0012 | 4.19 mg/mL | 0.0501 mg/mL | 37.37 units | 0.0506 mg/mL | 34.87 units |
| Red | |||||||
| Blue! | Ni-agarose | 0.6709x-0.0002 | 3.75mg/mL | 0.647 | 0.0318 | 0.322 | 0.233 |
| Pink | |||||||
| Yellow | paramagnetic beads | y = 1.11x-.06 | .55 mg/mL | .50 mg/mL | OD420=.56, Units = 48890.86 | .61 mg/mL | OD420=.63, Units = 34878.04 |
Wednesday/Friday
| Team Color | purification method | equation for BSA standard curve | concentration of beta-gal stock | concentration of purified beta-gal (wild type) | activity of purified beta-gal (wild type) | concentration of modified beta-gal (list modification below table) | activity of modified beta-gal |
|---|---|---|---|---|---|---|---|
| Green | |||||||
| Purple | |||||||
| Red | |||||||
| Blue | |||||||
| Pink | |||||||
| Yellow |
