Talk:BE.109:Systems engineering/Measuring DNA, RNA, protein: Difference between revisions

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Instructions: Copy this page (with the exception of the links at the bottom) to your user page (or to a page linked from your user page) and fill it out on the wiki for submission. 
==Record for experiment addressing Coli-roid operating conditions==
==Record for experiment addressing Coli-roid operating conditions==


===Lab section===
===Lab section===
''Circle one''
<!-- Choose either T/R or W/F --->


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===Team color===
===Team color===
''Circle one''
<!-- Choose one --->


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===Describe the experimental variations you are comparing.===
===Experiment variations===
<!-- Describe the experimental variations you are comparing. --->




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===What were the:===
===Sample densities===
<!-- Put the densities of your samples here --->


====density of your two samples?====






===Activity of samples===
<!-- Record the beta-galactosidase activity of your samples --->




====b-gal values measured for the two samples?====




 
===RNA concentrations===
 
<!-- concentrations of total RNA that you isolated from the two samples? --->
 
====concentrations of total RNA that you isolated from the two samples?====
 




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====Method of cDNA synthesis====
====Method of cDNA synthesis====
''Circle one''
<!-- Which method of cDNA synthesis did you use? --->


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===Primer pair used===
===Primer pair===
''Circle one''
<!-- Choose a primer pair --->


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[[Image:Macintosh HD-Users-nkuldell-Desktop-RTPCRplate.png| RT-PCR 96 well plate]]
[[Image:Macintosh HD-Users-nkuldell-Desktop-RTPCRplate.png| RT-PCR 96 well plate]]
==Online submissions==
<!-- Link the page with your RT-PCR reaction information here. --->
===Tues/Thurs section===
#[[User:Justinhlo]]
===Wed/Fri section===
#[[User:Danielle/3-5]]
#[[User:Tracyc/Talk:mod3day5]] & [[User:Yeem/Talk:mod3day5]]
#[[User_talk:tamijean]]
#[[User:Sashab|Sasha and Meghana are awesome]]

Latest revision as of 13:33, 26 April 2006

Instructions: Copy this page (with the exception of the links at the bottom) to your user page (or to a page linked from your user page) and fill it out on the wiki for submission.

Record for experiment addressing Coli-roid operating conditions

Lab section

T/R W/F

Team color

Green Purple Blue Red Pink Yellow

Experiment variations

Sample densities

Activity of samples

RNA concentrations

Method of cDNA synthesis

Epicenter Invitrogen

Primer pair

5' end of LacZ gene Middle of LacZ gene 3' end of LacZ

Wells for your samples:

RT-PCR 96 well plate

Online submissions

Tues/Thurs section

  1. User:Justinhlo

Wed/Fri section

  1. User:Danielle/3-5
  2. User:Tracyc/Talk:mod3day5 & User:Yeem/Talk:mod3day5
  3. User_talk:tamijean
  4. Sasha and Meghana are awesome
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