Talk:BE.109:Systems engineering/RT-PCR data analysis: Difference between revisions
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===Team Green=== | ===Team Green=== | ||
Because of outside factors, our group tested light and dark cells versus each other. Our standard was done with DNA from the lys-n-go reaction. The curve had an equation of y = -0.63x + 21.29, with an R^2 value of 0.633. The poor corelation can be attributed to the first measurement of the undiluted DNA. | Because of outside factors, our group tested light and dark cells versus each other. Our standard was done with DNA from the lys-n-go reaction. The curve had an equation of y = -0.63x + 21.29, with an R^2 value of 0.633. The poor corelation can be attributed to the first measurement of the undiluted DNA. | ||
===Team Pink!=== | |||
We varied cell density and culture volume by increasing cell density and lowering volume - the two factors worked against each other, which we did not plan well. This condition lowered beta-gal activity and lowered copies of lac Z in the cells, so the net effect of our varied conditions was to reduce the total number of cells in the sample. The curve from the standard DNA had an r squared value of 1 and an equation of y = -0.33x+13.03. This curve was made with two values, the undiluted sample and 1:10 dilution, which corresponded to 10,000 and 1,000 copies of lac z gene, respectively. | |||
==Wed/Fri section== | ==Wed/Fri section== | ||
Revision as of 13:25, 25 April 2006
Tues/Thurs section
Team Blue!
Our situation is special because we did two standard curves instead of one standard and data. One standard was done with DNA from lys-n-go reactions. One curve from standard conditions, and one curve from our variable condition. The condition we varied was the amount of air-flow allowed the cells. The standard culture was given air-flow, but the variable condition was completely sealed off in order to stress the cells. The curve from the standard air-flow condition had an r squared value of 0.8219, and equation of y = -0.35x + 12.44 The curve from the standard no air-flow condition had an r squared value of 1.000, and equation of y = -.37x + 13.22 Each curve was made with three values (the 1000, 100, 10 copies of LacZ)
Team Green
Because of outside factors, our group tested light and dark cells versus each other. Our standard was done with DNA from the lys-n-go reaction. The curve had an equation of y = -0.63x + 21.29, with an R^2 value of 0.633. The poor corelation can be attributed to the first measurement of the undiluted DNA.
Team Pink!
We varied cell density and culture volume by increasing cell density and lowering volume - the two factors worked against each other, which we did not plan well. This condition lowered beta-gal activity and lowered copies of lac Z in the cells, so the net effect of our varied conditions was to reduce the total number of cells in the sample. The curve from the standard DNA had an r squared value of 1 and an equation of y = -0.33x+13.03. This curve was made with two values, the undiluted sample and 1:10 dilution, which corresponded to 10,000 and 1,000 copies of lac z gene, respectively.
