Talk:BE.109:Systems engineering/RT-PCR data analysis

Tues/Thurs section

Team Blue!

Our situation is special because we did two standard curves instead of one standard and data. One standard was done with DNA from lys-n-go reactions. One curve from standard conditions, and one curve from our variable condition. The condition we varied was the amount of air-flow allowed the cells. The standard culture was given air-flow, but the variable condition was completely sealed off in order to stress the cells. The curve from the standard air-flow condition had an r squared value of 0.8219, and equation of y = -0.35x + 12.44 The curve from the standard no air-flow condition had an r squared value of 1.000, and equation of y = -.37x + 13.22 Each curve was made with three values (the 1000, 100, 10 copies of LacZ)