Talk:Beta-Galactosidase Assay (A better Miller)

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Revision as of 12:30, 15 October 2007 by Reshma P. Shetty (Talk | contribs)
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  • Reshma 18:55, 17 September 2007 (EDT): It would be useful to know how long the permeabilization, substrate and stop solutions keep. Presumably, since the permeabilization and substrate solutions each contains β-mercaptoethanol, they need to be made fresh each time?
    • Reshma 11:30, 15 October 2007 (CDT): The substrate solution is traditionally made fresh each day because solutions with ONPG only keep for a few days at 4°C.
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