Talk:CH391L/S12/GeneandGenomeSynthesis

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*'''[[User:Jeffrey E. Barrick|Jeffrey E. Barrick]] 11:17, 14 February 2012 (EST)''':You mentioned the [http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Cloning/gene-synthesis.html Gene Art kits from Invitrogen] in class. They advertise a lot of applications for stitching together up to ten fragments at a time. I guess no one really knows what's in the kits though?
*'''[[User:Jeffrey E. Barrick|Jeffrey E. Barrick]] 11:17, 14 February 2012 (EST)''':You mentioned the [http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Cloning/gene-synthesis.html Gene Art kits from Invitrogen] in class. They advertise a lot of applications for stitching together up to ten fragments at a time. I guess no one really knows what's in the kits though?
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**'''[[User:Joe Hanson|Joe Hanson]] 15:26, 15 February 2012 (EST)''': Judging from the references in the end of their manual for the [http://tools.invitrogen.com/content/sfs/manuals/geneart_seamless_cloning_and_assembly_man.pdf GeneArt Seamless Assembly Kit], I can gather that it's an ''in vitro'' recombination system derived from a some or all parts of a few [http://en.wikipedia.org/wiki/Recombineering recombineering] methods. For those who care to look it up, in the tube, you probably get a mix of [http://www.ncbi.nlm.nih.gov/pubmed/9500923 Lambda Redβ protein], E. coli [http://www.ncbi.nlm.nih.gov/pubmed/9771703 RecE/RecT proteins] and [http://www.ncbi.nlm.nih.gov/pubmed/11118378 vaccinia virus DNA polymerase].
*'''[[User:Michael Hammerling|Michael Hammerling]] 14:50, 15 February 2012 (EST)''': What types of sequences are particularly difficult to synthesize using these methods?
*'''[[User:Michael Hammerling|Michael Hammerling]] 14:50, 15 February 2012 (EST)''': What types of sequences are particularly difficult to synthesize using these methods?

Revision as of 15:26, 15 February 2012


  • Jeffrey E. Barrick 11:17, 14 February 2012 (EST):You mentioned the Gene Art kits from Invitrogen in class. They advertise a lot of applications for stitching together up to ten fragments at a time. I guess no one really knows what's in the kits though?
  • Michael Hammerling 14:50, 15 February 2012 (EST): What types of sequences are particularly difficult to synthesize using these methods?
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