Talk:CH391L/S12/GeneandGenomeSynthesis
From OpenWetWare
Jump to navigationJump to search
- Jeffrey E. Barrick 11:17, 14 February 2012 (EST):You mentioned the Gene Art kits from Invitrogen in class. They advertise a lot of applications for stitching together up to ten fragments at a time. I guess no one really knows what's in the kits though?
- Joe Hanson 15:26, 15 February 2012 (EST): Judging from the references in the end of their manual for the GeneArt Seamless Assembly Kit, I can gather that it's an in vitro recombination system derived from a some or all parts of a few recombineering methods. For those who care to look it up, in the tube, you probably get a mix of Lambda Redβ protein, E. coli RecE/RecT proteins and vaccinia virus DNA polymerase.
- Michael Hammerling 14:50, 15 February 2012 (EST): What types of sequences are particularly difficult to synthesize using these methods?
