Talk:CH391L/S12/In vitro Selection: Difference between revisions
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How is it that you can isolate specific protein or gene products in oil bubbles in an emulsion of oil and water? --[[User:Midhat Patel|mpatel927@gmail.com]] 16:53, 31 January 2012 (EST) | How is it that you can isolate specific protein or gene products in oil bubbles in an emulsion of oil and water? --[[User:Midhat Patel|mpatel927@gmail.com]] 16:53, 31 January 2012 (EST) | ||
*'''[[User:Jeffrey E. Barrick|Jeffrey E. Barrick]] 11:32, 5 February 2012 (EST)''':Overall organization is good. It would be nice to have a better introduction at the beginning of this topic. It jumps right into details without explaining where it's going. | |||
*'''[[User:Jeffrey E. Barrick|Jeffrey E. Barrick]] 11:32, 5 February 2012 (EST)''':I think all the schemes are "Selective Amplification" and that the ribozyme scheme might be better called "Self-modification"? | |||
*'''[[User:Jeffrey E. Barrick|Jeffrey E. Barrick]] 11:32, 5 February 2012 (EST)''':To flesh out some important but neglected sections, you should really add at least one directed evolution reference (for a plasmid library selected in cells for a function). Maybe a Frances Arnold study where they change enzyme substrate specificity or thermostability? | |||
Revision as of 09:32, 5 February 2012
Concerning that in vitro compartmentalisation, if say, applied for measuring enzymatic functions, would that oil compound affect the activity of the enzyme? how would you control the inside temperature, and would that temperature change cause a change in the size of the compartment(since CMC depends on temp)?*Yi Kou 13:04, 31 January 2012 (EST):
How is it that you can isolate specific protein or gene products in oil bubbles in an emulsion of oil and water? --mpatel927@gmail.com 16:53, 31 January 2012 (EST)
- Jeffrey E. Barrick 11:32, 5 February 2012 (EST):Overall organization is good. It would be nice to have a better introduction at the beginning of this topic. It jumps right into details without explaining where it's going.
- Jeffrey E. Barrick 11:32, 5 February 2012 (EST):I think all the schemes are "Selective Amplification" and that the ribozyme scheme might be better called "Self-modification"?
- Jeffrey E. Barrick 11:32, 5 February 2012 (EST):To flesh out some important but neglected sections, you should really add at least one directed evolution reference (for a plasmid library selected in cells for a function). Maybe a Frances Arnold study where they change enzyme substrate specificity or thermostability?
