Talk:CH391L/S12/In vitro Selection

Concerning that in vitro compartmentalisation, if say, applied for measuring enzymatic functions, would that oil compound affect the activity of the enzyme? how would you control the inside temperature, and would that temperature change cause a change in the size of the compartment(since CMC depends on temp)?*Yi Kou 13:04, 31 January 2012 (EST):

How is it that you can isolate specific protein or gene products in oil bubbles in an emulsion of oil and water? --mpatel927@gmail.com 16:53, 31 January 2012 (EST)

• Jeffrey E. Barrick 11:32, 5 February 2012 (EST):Overall organization is good. It would be nice to have a better introduction at the beginning of this topic. It jumps right into details without explaining where it's going.
• Jeffrey E. Barrick 11:32, 5 February 2012 (EST):I think all the schemes are "Selective Amplification" and that the ribozyme scheme might be better called "Self-modification"?
• Jeffrey E. Barrick 11:32, 5 February 2012 (EST):To flesh out some important but neglected sections, you should really add at least one directed evolution reference (for a plasmid library selected in cells for a function). Maybe a Frances Arnold study where they change enzyme substrate specificity or thermostability?

• Erik Quandt 22:50, 5 February 2012 (EST): I thought that phage display deserved a mention
• Adam Meyer 17:35, 9 February 2012 (EST):I thought that selecting for protein-binding (including phage display) was too big a topic to cover. Although it is arguably the most successful facet of in vitro selection.

• David M. Truong 23:03, 5 February 2012 (EST): I'm curious about what you do when your in vitro selected product doesn't work in vivo (or at least not as well)? How do the limitations of in vitro selection go hand-in-hand with in vivo selection experiments, and vice versa?
• Adam Meyer 17:35, 9 February 2012 (EST):It is common for selected variants to be optimized to function in the selection (and not where you actually want to use it). The best thing you can do is design

your selection scheme to closely mimic the conditions in which the final product will be used. You get what you select for.