Talk:CH391L/S12/Locomotion

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*'''[[User:Joe Hanson|Joe Hanson]] 12:17, 26 March 2012 (EDT)''':In addition to the "run state" phenotypes, are there any known genetic mutations that provide intermediate <i>E. coli</i> flagellar activity? Are there any "slow" flagellar mutations or dominant negatives known? I am certainly not asking this as a way to get help with our group's class project.
*'''[[User:Joe Hanson|Joe Hanson]] 12:17, 26 March 2012 (EDT)''':In addition to the "run state" phenotypes, are there any known genetic mutations that provide intermediate <i>E. coli</i> flagellar activity? Are there any "slow" flagellar mutations or dominant negatives known? I am certainly not asking this as a way to get help with our group's class project.
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**--[[User:Erik Quandt|Erik Quandt]] 10:30, 2 April 2012 (EDT):  There have been several igem projects aimed at controlling flagellar activity.  The [http://2010.igem.org/Team:SDU-Denmark/project-t#Hyperflagellation 2010 Denmark Team] sought to produce a "hyperflagellated" that would produce more flagella and be more motile.  They did this by overexpressing the flgDC operon, flgDC regulates the expression of genes required for flagellar assembly.  Other groups have tried to control the expression of motor proteins ''MotA'' and ''MotB'' in order to control the rotation of the flagella in response to quorum sensing signals [http://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=4290622&userType=inst bacterial relay race].
*'''[[User:Jeffrey E. Barrick|Jeffrey E. Barrick]] 17:42, 1 April 2012 (EDT)''':Here's a [http://2010.igem.org/Team:ETHZ_Basel 2010 ETHZ Basel iGEM project] where they use light to control tumbling versus running.
*'''[[User:Jeffrey E. Barrick|Jeffrey E. Barrick]] 17:42, 1 April 2012 (EDT)''':Here's a [http://2010.igem.org/Team:ETHZ_Basel 2010 ETHZ Basel iGEM project] where they use light to control tumbling versus running.

Revision as of 10:30, 2 April 2012

  • Joe Hanson 12:17, 26 March 2012 (EDT):In addition to the "run state" phenotypes, are there any known genetic mutations that provide intermediate E. coli flagellar activity? Are there any "slow" flagellar mutations or dominant negatives known? I am certainly not asking this as a way to get help with our group's class project.
    • --Erik Quandt 10:30, 2 April 2012 (EDT): There have been several igem projects aimed at controlling flagellar activity. The 2010 Denmark Team sought to produce a "hyperflagellated" that would produce more flagella and be more motile. They did this by overexpressing the flgDC operon, flgDC regulates the expression of genes required for flagellar assembly. Other groups have tried to control the expression of motor proteins MotA and MotB in order to control the rotation of the flagella in response to quorum sensing signals bacterial relay race.
  • Jeffrey E. Barrick 17:49, 1 April 2012 (EDT): Listeria's form of locomotion is the coolest I've seen. It zooms around like a comet inside mammalian cells by hijacking actin polymerization (the same mechanism by which amoeba and white blood cells crawl around).
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