Talk:CH391L/S12/Locomotion

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
*'''[[User:Joe Hanson|Joe Hanson]] 12:17, 26 March 2012 (EDT)''':In addition to the "run state" phenotypes, are there any known genetic mutations that provide intermediate <i>E. coli</i> flagellar activity? Are there any "slow" flagellar mutations or dominant negatives known? I am certainly not asking this as a way to get help with our group's class project.
*'''[[User:Joe Hanson|Joe Hanson]] 12:17, 26 March 2012 (EDT)''':In addition to the "run state" phenotypes, are there any known genetic mutations that provide intermediate <i>E. coli</i> flagellar activity? Are there any "slow" flagellar mutations or dominant negatives known? I am certainly not asking this as a way to get help with our group's class project.
-
**--[[User:Erik Quandt|Erik Quandt]] 10:30, 2 April 2012 (EDT):  There have been several igem projects aimed at controlling flagellar activity.  The [http://2010.igem.org/Team:SDU-Denmark/project-t#Hyperflagellation 2010 Denmark Team] sought to produce a "hyperflagellated" that would produce more flagella and be more motile.  They did this by overexpressing the flgDC operon, flgDC regulates the expression of genes required for flagellar assembly.  Perhaps "slow" cells could be engineered by reducing the number of flagella produced?  Other groups have tried to control the expression of the motor protein ''MotB'' in order to control the rotation of the flagella in response to quorum sensing signals [http://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=4290622&userType=inst bacterial relay race].  ''Bacillus subtilis'' has a protein ''EpsE'' that acts as a flagellar "clutch" which regulates the rotation of the flagella.
+
**--[[User:Erik Quandt|Erik Quandt]] 10:30, 2 April 2012 (EDT):  There have been several igem projects aimed at controlling flagellar activity.  The [http://2010.igem.org/Team:SDU-Denmark/project-t#Hyperflagellation 2010 Denmark Team] sought to produce a "hyperflagellated" that would produce more flagella and be more motile.  They did this by overexpressing the flgDC operon, flgDC regulates the expression of genes required for flagellar assembly.  Perhaps "slow" cells could be engineered by reducing the number of flagella produced?  Other groups have tried to control the expression of the motor protein ''MotB'' in order to control the rotation of the flagella in response to quorum sensing signals [http://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=4290622&userType=inst bacterial relay race].  ''Bacillus subtilis'' has a protein ''EpsE'' that acts as a flagellar "clutch" which regulates the rotation of the flagella .
*'''[[User:Jeffrey E. Barrick|Jeffrey E. Barrick]] 17:42, 1 April 2012 (EDT)''':Here's a [http://2010.igem.org/Team:ETHZ_Basel 2010 ETHZ Basel iGEM project] where they use light to control tumbling versus running.
*'''[[User:Jeffrey E. Barrick|Jeffrey E. Barrick]] 17:42, 1 April 2012 (EDT)''':Here's a [http://2010.igem.org/Team:ETHZ_Basel 2010 ETHZ Basel iGEM project] where they use light to control tumbling versus running.
Line 8: Line 8:
*'''[[User:Jeffrey E. Barrick|Jeffrey E. Barrick]] 17:49, 1 April 2012 (EDT)''': Listeria's form of locomotion is the coolest I've seen. [http://www.youtube.com/watch?v=sF4BeU60yT8 It zooms around like a comet] inside mammalian cells by hijacking actin polymerization (the same mechanism by which amoeba and white blood cells crawl around).
*'''[[User:Jeffrey E. Barrick|Jeffrey E. Barrick]] 17:49, 1 April 2012 (EDT)''': Listeria's form of locomotion is the coolest I've seen. [http://www.youtube.com/watch?v=sF4BeU60yT8 It zooms around like a comet] inside mammalian cells by hijacking actin polymerization (the same mechanism by which amoeba and white blood cells crawl around).
 +
 +
=References=
 +
<biblio>
 +
#Blair2008 pmid=18566286

Revision as of 10:36, 2 April 2012

  • Joe Hanson 12:17, 26 March 2012 (EDT):In addition to the "run state" phenotypes, are there any known genetic mutations that provide intermediate E. coli flagellar activity? Are there any "slow" flagellar mutations or dominant negatives known? I am certainly not asking this as a way to get help with our group's class project.
    • --Erik Quandt 10:30, 2 April 2012 (EDT): There have been several igem projects aimed at controlling flagellar activity. The 2010 Denmark Team sought to produce a "hyperflagellated" that would produce more flagella and be more motile. They did this by overexpressing the flgDC operon, flgDC regulates the expression of genes required for flagellar assembly. Perhaps "slow" cells could be engineered by reducing the number of flagella produced? Other groups have tried to control the expression of the motor protein MotB in order to control the rotation of the flagella in response to quorum sensing signals bacterial relay race. Bacillus subtilis has a protein EpsE that acts as a flagellar "clutch" which regulates the rotation of the flagella .
  • Jeffrey E. Barrick 17:49, 1 April 2012 (EDT): Listeria's form of locomotion is the coolest I've seen. It zooms around like a comet inside mammalian cells by hijacking actin polymerization (the same mechanism by which amoeba and white blood cells crawl around).

References

  1. Blair KM, Turner L, Winkelman JT, Berg HC, and Kearns DB. . pmid:18566286. PubMed HubMed [Blair2008]
Personal tools