Talk:CH391L/S12/MAGE lycopene production, CAGE "Amberless" E. coli

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Revision as of 10:13, 14 April 2012 by Jeffrey E. Barrick (talk | contribs) (New page: *'''~~~~''':There's an NAR paper about using modified bases in the oligos to avoid having to knock out MMR repair to get high efficiency incorporation. Can you summarize? <cite>Wang2011</c...)
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  • Jeffrey E. Barrick 13:13, 14 April 2012 (EDT):There's an NAR paper about using modified bases in the oligos to avoid having to knock out MMR repair to get high efficiency incorporation. Can you summarize? [1].
  1. Wang HH, Xu G, Vonner AJ, and Church G. Modified bases enable high-efficiency oligonucleotide-mediated allelic replacement via mismatch repair evasion. Nucleic Acids Res. 2011 Sep 1;39(16):7336-47. DOI:10.1093/nar/gkr183 | PubMed ID:21609953 | HubMed [Wang2011]

    Modified bases enable high-efficiency oligonucleotide-mediated allelic replacement via mismatch repair evasion.