Talk:CH391L/S12/PCR and advanced PCR techniques

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 2: Line 2:
:*'''[[User:Yi Kou|Yi Kou]] 18:24, 6 February 2012 (EST)''':Really nice technique!
:*'''[[User:Yi Kou|Yi Kou]] 18:24, 6 February 2012 (EST)''':Really nice technique!
*[[User:Razan Alnahhas|Razan Alnahhas]] 23:12, 9 February 2012 (EST): Has PCR mediated DNA shuffling been successfully used in directed evolution, and are there only certain sequences that this technique can work on?
*[[User:Razan Alnahhas|Razan Alnahhas]] 23:12, 9 February 2012 (EST): Has PCR mediated DNA shuffling been successfully used in directed evolution, and are there only certain sequences that this technique can work on?
-
 
+
*'''[[User:Ben Slater|Ben Slater]] 12:28, 10 February 2012 (EST)''': Hi Yi, I noticed you mentioned a few things about Phusion being both more accurate and faster than Taq. So what is the downside? Price? Does it have any different thermal sensitivity?

Revision as of 13:28, 10 February 2012

  • Brian Renda 15:47, 6 February 2012 (EST): Another cool PCR variant is called RPA (recombinase polymerase amplification), it is isothermal, finished in about 10-15 minutes, and the ingredients can be dried and stored at room temperature [1].
  • Yi Kou 18:24, 6 February 2012 (EST):Really nice technique!
  • Razan Alnahhas 23:12, 9 February 2012 (EST): Has PCR mediated DNA shuffling been successfully used in directed evolution, and are there only certain sequences that this technique can work on?
  • Ben Slater 12:28, 10 February 2012 (EST): Hi Yi, I noticed you mentioned a few things about Phusion being both more accurate and faster than Taq. So what is the downside? Price? Does it have any different thermal sensitivity?


References

Error fetching PMID 16756388:
  1. Error fetching PMID 16756388: [Piepenburg2006]
Personal tools