Talk:CH391L/S12/PCR and advanced PCR techniques: Difference between revisions
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:*'''[[User:Yi Kou|Yi Kou]] 18:24, 6 February 2012 (EST)''':Really nice technique! | :*'''[[User:Yi Kou|Yi Kou]] 18:24, 6 February 2012 (EST)''':Really nice technique! | ||
*[[User:Razan Alnahhas|Razan Alnahhas]] 23:12, 9 February 2012 (EST): Has PCR mediated DNA shuffling been successfully used in directed evolution, and are there only certain sequences that this technique can work on? | *[[User:Razan Alnahhas|Razan Alnahhas]] 23:12, 9 February 2012 (EST): Has PCR mediated DNA shuffling been successfully used in directed evolution, and are there only certain sequences that this technique can work on? | ||
*'''[[User:Ben Slater|Ben Slater]] 12:28, 10 February 2012 (EST)''': Hi Yi, I noticed you mentioned a few things about Phusion being both more accurate and faster than Taq. So what is the downside? Price? Does it have any different thermal sensitivity? | |||
Revision as of 10:28, 10 February 2012
- Brian Renda 15:47, 6 February 2012 (EST): Another cool PCR variant is called RPA (recombinase polymerase amplification), it is isothermal, finished in about 10-15 minutes, and the ingredients can be dried and stored at room temperature [1].
- Yi Kou 18:24, 6 February 2012 (EST):Really nice technique!
- Razan Alnahhas 23:12, 9 February 2012 (EST): Has PCR mediated DNA shuffling been successfully used in directed evolution, and are there only certain sequences that this technique can work on?
- Ben Slater 12:28, 10 February 2012 (EST): Hi Yi, I noticed you mentioned a few things about Phusion being both more accurate and faster than Taq. So what is the downside? Price? Does it have any different thermal sensitivity?
