Talk:CH391L/S12/Protein regulators of transcription: Difference between revisions
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*'''[[User:Jeffrey E. Barrick|Jeffrey E. Barrick]] 12:18, 7 March 2012 (EST)''':There is one distinction between Lac and Ara regulation that is important. Then the regulatory protein is absent in the Lac system (for example, missing from a strain), your promoter is constitutively ON. Then the regulatory protein from the Ara system is absent, your promoter is constitutively OFF. These states will also result if a mutation happens that knocks out the regulatory protein, which can make the systems differentially robust to mutations. | *'''[[User:Jeffrey E. Barrick|Jeffrey E. Barrick]] 12:18, 7 March 2012 (EST)''':There is one distinction between Lac and Ara regulation that is important. Then the regulatory protein is absent in the Lac system (for example, missing from a strain), your promoter is constitutively ON. Then the regulatory protein from the Ara system is absent, your promoter is constitutively OFF. These states will also result if a mutation happens that knocks out the regulatory protein, which can make the systems differentially robust to mutations. | ||
*'''[[User:Yi Kou|Yi Kou]] 12:42, 19 March 2012 (EDT)''': sorry for adding this late, are there any studies on leaky expression of lac and ara operons? I am kind of interested in how they quantify this effect. | |||
Latest revision as of 09:42, 19 March 2012
- Brian Renda 17:23, 5 March 2012 (EST):to add: gal, tet on, off, copper, m-toluic acid
- Ben Slater 02:28, 19 March 2012 (EDT): Updated.
- Jeffrey E. Barrick 12:18, 7 March 2012 (EST):There is one distinction between Lac and Ara regulation that is important. Then the regulatory protein is absent in the Lac system (for example, missing from a strain), your promoter is constitutively ON. Then the regulatory protein from the Ara system is absent, your promoter is constitutively OFF. These states will also result if a mutation happens that knocks out the regulatory protein, which can make the systems differentially robust to mutations.
- Yi Kou 12:42, 19 March 2012 (EDT): sorry for adding this late, are there any studies on leaky expression of lac and ara operons? I am kind of interested in how they quantify this effect.
