Talk:CH391L/S12/Refactoring Genomes: Difference between revisions
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*'''[[User:Jeffrey E. Barrick|Jeffrey E. Barrick]] 17:01, 2 April 2012 (EDT)''':Did they replace the telomeres? With what? | *'''[[User:Jeffrey E. Barrick|Jeffrey E. Barrick]] 17:01, 2 April 2012 (EDT)''':Did they replace the telomeres? With what? | ||
*'''[[User:Jeffrey E. Barrick|Jeffrey E. Barrick]] 10:11, 6 April 2012 (EDT)''':General note: I don't like the trend of pasting summary boxes from papers into these Wiki pages. The point of making this page is to digest information into important points and bring together different papers, not reproduce existing information. You already have much of the information in your description, anyway. | |||
*'''[[User:Jeffrey E. Barrick|Jeffrey E. Barrick]] 10:11, 6 April 2012 (EDT)''':Why don't you cite a paper from sequencing T7? | |||
*'''[[User:Jeffrey E. Barrick|Jeffrey E. Barrick]] 10:11, 6 April 2012 (EDT)''':Can you describe in more detail the method of genome construction they used for T7? They put the segment in a plasmid and changed it, or they PCRed each gene with primers that changed the endpoints like you have pictured? How do they recombine the genomes? Is it by infecting a cell carrying a plasmid with wild-type T7 and screening for recombinants? | |||
*'''[[User:Jeffrey E. Barrick|Jeffrey E. Barrick]] 10:11, 6 April 2012 (EDT)''':There is a 2009 book on genome refactoring that is available for [http://catalog.lib.utexas.edu/search/X?SEARCH=genome+refactoring online reading through the library]. It describes refactoring the phage M13 genome in an undergraduate lab course at MIT, and actually has some other sections of general interest for teaching synthetic biology. | |||
Revision as of 07:11, 6 April 2012
- Jeffrey E. Barrick 17:01, 2 April 2012 (EDT):Did they replace the telomeres? With what?
- Jeffrey E. Barrick 10:11, 6 April 2012 (EDT):General note: I don't like the trend of pasting summary boxes from papers into these Wiki pages. The point of making this page is to digest information into important points and bring together different papers, not reproduce existing information. You already have much of the information in your description, anyway.
- Jeffrey E. Barrick 10:11, 6 April 2012 (EDT):Why don't you cite a paper from sequencing T7?
- Jeffrey E. Barrick 10:11, 6 April 2012 (EDT):Can you describe in more detail the method of genome construction they used for T7? They put the segment in a plasmid and changed it, or they PCRed each gene with primers that changed the endpoints like you have pictured? How do they recombine the genomes? Is it by infecting a cell carrying a plasmid with wild-type T7 and screening for recombinants?
- Jeffrey E. Barrick 10:11, 6 April 2012 (EDT):There is a 2009 book on genome refactoring that is available for online reading through the library. It describes refactoring the phage M13 genome in an undergraduate lab course at MIT, and actually has some other sections of general interest for teaching synthetic biology.
