Talk:CH391L/S12/Restriction enzymes and BioBricks assembly standards: Difference between revisions
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Minor note about endonuclease nomenclature: Endonucleases like I-SceI are not really "restriction endonucleases" because they do not originate from the bacterial restriction system. They are "homing endonucleases" from yeast ("S"accharomyces "ce"revisiae) that help introns move around in yeast genomes. There's lots of other members of this family that have large sites like I-SceI and they are useful, but they aren't "restriction" endonucleases. *'''[[User:Joe Hanson|Joe Hanson]] 15:18, 6 February 2012 (EST)''': | Minor note about endonuclease nomenclature: Endonucleases like I-SceI are not really "restriction endonucleases" because they do not originate from the bacterial restriction system. They are "homing endonucleases" from yeast ("S"accharomyces "ce"revisiae) that help introns move around in yeast genomes. There's lots of other members of this family that have large sites like I-SceI and they are useful, but they aren't "restriction" endonucleases. *'''[[User:Joe Hanson|Joe Hanson]] 15:18, 6 February 2012 (EST)''': | ||
*'''[[User:David M. Truong|David M. Truong]] 15:26, 6 February 2012 (EST)''':You may want to define more clearly what you mean by scar. Simple definition might be the ligation of two different restriction sticky ends, that remove the palindrome, and therefore can no longer be used as a restriction site. Also, you might also discuss the vast commercial sector for restriction enzymes, most importantly, New England Biolabs. | |||
Revision as of 13:26, 6 February 2012
Minor note about endonuclease nomenclature: Endonucleases like I-SceI are not really "restriction endonucleases" because they do not originate from the bacterial restriction system. They are "homing endonucleases" from yeast ("S"accharomyces "ce"revisiae) that help introns move around in yeast genomes. There's lots of other members of this family that have large sites like I-SceI and they are useful, but they aren't "restriction" endonucleases. *Joe Hanson 15:18, 6 February 2012 (EST):
- David M. Truong 15:26, 6 February 2012 (EST):You may want to define more clearly what you mean by scar. Simple definition might be the ligation of two different restriction sticky ends, that remove the palindrome, and therefore can no longer be used as a restriction site. Also, you might also discuss the vast commercial sector for restriction enzymes, most importantly, New England Biolabs.
