Talk:CH391L/S12/SpinachRNA: Difference between revisions
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*'''[[User:Peter Otoupal|Peter Otoupal]] 22:33, 1 March 2012 (EST)''':Is it possible to use this chromophore-aptamer complex fluorescence technique to distinguish localized sites of RNA activity in cells? [http://openwetware.org/images/2/2e/Ecoli_SpA.png This picture] makes it seem like the entire <i>E. coli</i> cell is caused to fluoresce when Spinach is expressed. It seems like a great technique; I guess it just seems like Spinach's strength would be in pinpointing RNA activity within cells, and it's hard to tell if such precision is possible from that picture. | *'''[[User:Peter Otoupal|Peter Otoupal]] 22:33, 1 March 2012 (EST)''':Is it possible to use this chromophore-aptamer complex fluorescence technique to distinguish localized sites of RNA activity in cells? [http://openwetware.org/images/2/2e/Ecoli_SpA.png This picture] makes it seem like the entire <i>E. coli</i> cell is caused to fluoresce when Spinach is expressed. It seems like a great technique; I guess it just seems like Spinach's strength would be in pinpointing RNA activity within cells, and it's hard to tell if such precision is possible from that picture. | ||
**'''[[User:David M. Truong|David M. Truong]] 22:50, 1 March 2012 (EST)''':Going with what Peter said, what is its potential for single-molecule use? Is the signal too diffuse (and the background too high)? Does it have potential for calculateing the "center" of the fluorescence emission and determine single molecules like when using | **'''[[User:David M. Truong|David M. Truong]] 22:50, 1 March 2012 (EST)''':Going with what Peter said, what is its potential for single-molecule use? Is the signal too diffuse (and the background too high)? Does it have potential for calculateing the "center" of the fluorescence emission and determine single molecules like when using GFP? | ||
Revision as of 20:50, 1 March 2012
- Peter Otoupal 22:33, 1 March 2012 (EST):Is it possible to use this chromophore-aptamer complex fluorescence technique to distinguish localized sites of RNA activity in cells? This picture makes it seem like the entire E. coli cell is caused to fluoresce when Spinach is expressed. It seems like a great technique; I guess it just seems like Spinach's strength would be in pinpointing RNA activity within cells, and it's hard to tell if such precision is possible from that picture.
- David M. Truong 22:50, 1 March 2012 (EST):Going with what Peter said, what is its potential for single-molecule use? Is the signal too diffuse (and the background too high)? Does it have potential for calculateing the "center" of the fluorescence emission and determine single molecules like when using GFP?
