Talk:CH391L/S12/TranscriptionPromotersandTerminators: Difference between revisions
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*'''[[User:Jeffrey E. Barrick|Jeffrey E. Barrick]] 11:16, 3 March 2012 (EST)''':How did they create the "insulator" sequence that didn't have any known transcription factor binding sites? Did they make it up from scratch, or did they grab it from somewhere else in a different genome? | *'''[[User:Jeffrey E. Barrick|Jeffrey E. Barrick]] 11:16, 3 March 2012 (EST)''':How did they create the "insulator" sequence that didn't have any known transcription factor binding sites? Did they make it up from scratch, or did they grab it from somewhere else in a different genome? | ||
**'''[[User:Yi Kou|Yi Kou]] 11:49, 3 March 2012 (EST)''':for the insulator | **'''[[User:Yi Kou|Yi Kou]] 11:49, 3 March 2012 (EST)''':for the insulator, one way to detect <cite>p3</cite>. | ||
=References= | =References= | ||
<biblio> | <biblio> | ||
#paper1 pmid=16352826 | #paper1 pmid=16352826 | ||
#paper2 pmid=11525731 | #paper2 pmid=11525731 | ||
#p3 pmid=12154228 | |||
</biblio> | </biblio> | ||
Revision as of 09:51, 3 March 2012
- Midhat Patel 15:50, 27 February 2012 (EST): Can sigma factors be used to regulate gene expression? If we use a promoter associated with a sigma factor that operates under specific conditions, can it be used as a promoter for a system and integrated into E. coli?
- Yi Kou 10:07, 3 March 2012 (EST): Good idea! Here is one paper of similar "genetic transplantation" of sigma factor[1]. But I think for actual application, it is not so simple as one factor and binding sequence could do the work. Many regulations, involving posttranslational regulation and auto-regulation have been confirmed for many types of sigma factors. And if these accompanies are not well considered, I think it would be hard to observe the expected result or phenotype. Also, it has been suggested that the sigma factor is not "on and off" the core pol but rather shows a "self-changing" pattern associated with the RNA pol[2]. This might add another influence of "interaction spectrum" with this sigma factor "transplantation" idea.
- Jeffrey E. Barrick 11:16, 3 March 2012 (EST):How did they create the "insulator" sequence that didn't have any known transcription factor binding sites? Did they make it up from scratch, or did they grab it from somewhere else in a different genome?
References
- Karlinsey JE and Hughes KT. Genetic transplantation: Salmonella enterica serovar Typhimurium as a host to study sigma factor and anti-sigma factor interactions in genetically intractable systems. J Bacteriol. 2006 Jan;188(1):103-14. DOI:10.1128/JB.188.1.103-114.2006 |
- Mukhopadhyay J, Kapanidis AN, Mekler V, Kortkhonjia E, Ebright YW, and Ebright RH. Translocation of sigma(70) with RNA polymerase during transcription: fluorescence resonance energy transfer assay for movement relative to DNA. Cell. 2001 Aug 24;106(4):453-63. DOI:10.1016/s0092-8674(01)00464-0 |
- Burgess-Beusse B, Farrell C, Gaszner M, Litt M, Mutskov V, Recillas-Targa F, Simpson M, West A, and Felsenfeld G. The insulation of genes from external enhancers and silencing chromatin. Proc Natl Acad Sci U S A. 2002 Dec 10;99 Suppl 4(Suppl 4):16433-7. DOI:10.1073/pnas.162342499 |
