Talk:CH391L/S12/TranslationRBSandCodons: Difference between revisions
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*'''[[User:Yi Kou|Yi Kou]] 08:59, 3 March 2012 (EST)''':some starins are engineered to express higher level of rare condons, such as Rosetta from Novagen, or BL21 condon plus from Stratagene. | *'''[[User:Yi Kou|Yi Kou]] 08:59, 3 March 2012 (EST)''':some starins are engineered to express higher level of rare condons, such as Rosetta from Novagen[http://www.med.unc.edu/csb/PEP/manuals/novagen_competent_cells2.pdf Rosetta competent cell from Novagen page5], or BL21 condon plus from Stratagene [http://www.med.unc.edu/csb/PEP/manuals/codon_plus_manual.pdf BL21 codon plus from Stratagene]. | ||
**'''[[User:Jeffrey E. Barrick|Jeffrey E. Barrick]] 10:17, 3 March 2012 (EST)''':That's a great area to follow up on! Can you research some of the things they have done in those strains to make them better at expressing proteins with rare codons. | **'''[[User:Jeffrey E. Barrick|Jeffrey E. Barrick]] 10:17, 3 March 2012 (EST)''':That's a great area to follow up on! Can you research some of the things they have done in those strains to make them better at expressing proteins with rare codons. | ||
*'''[[User:Jeffrey E. Barrick|Jeffrey E. Barrick]] 10:17, 3 March 2012 (EST)''':It seems like it would be a good idea to have a picture or link to the bacterial genetic code, in addition to the human one, since that's more likely to be of use in designing things for ''E. coli''. Ah... see you already have such a link. | *'''[[User:Jeffrey E. Barrick|Jeffrey E. Barrick]] 10:17, 3 March 2012 (EST)''':It seems like it would be a good idea to have a picture or link to the bacterial genetic code, in addition to the human one, since that's more likely to be of use in designing things for ''E. coli''. Ah... see you already have such a link. | ||
Revision as of 09:14, 3 March 2012
- Yi Kou 08:59, 3 March 2012 (EST):some starins are engineered to express higher level of rare condons, such as Rosetta from NovagenRosetta competent cell from Novagen page5, or BL21 condon plus from Stratagene BL21 codon plus from Stratagene.
- Jeffrey E. Barrick 10:17, 3 March 2012 (EST):That's a great area to follow up on! Can you research some of the things they have done in those strains to make them better at expressing proteins with rare codons.
- Jeffrey E. Barrick 10:17, 3 March 2012 (EST):It seems like it would be a good idea to have a picture or link to the bacterial genetic code, in addition to the human one, since that's more likely to be of use in designing things for E. coli. Ah... see you already have such a link.
