Talk:CH391L/S12/TranslationRBSandCodons

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Revision as of 18:05, 4 March 2012 by Michael Hammerling (Talk | contribs)
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  • Yi Kou 08:59, 3 March 2012 (EST):some starins are engineered to express higher level of rare condons, such as Rosetta from Novagen Rosetta competent cell from Novagen page5, or BL21 condon plus from Stratagene BL21 codon plus from Stratagene.
    • Jeffrey E. Barrick 10:17, 3 March 2012 (EST):That's a great area to follow up on! Can you research some of the things they have done in those strains to make them better at expressing proteins with rare codons.
  • Jeffrey E. Barrick 10:17, 3 March 2012 (EST):It seems like it would be a good idea to have a picture or link to the bacterial genetic code, in addition to the human one, since that's more likely to be of use in designing things for E. coli. Ah... see you already have such a link.
  • Ben Slater 16:09, 3 March 2012 (EST): You might want to add some info on how eukaryotic differs from prokaryotic translation, namely the eukaryotic initiation complex with the PAB (poly(a)-binding protein) and the scanning from the 5' cap to the first methionine instead of Shine-Dalgarno sequence recognition.
    • Ben Slater 16:22, 3 March 2012 (EST): I guess what I'm saying is that the 5' cap and the poly(A) tail are both unique to eukaryotic mRNA and both features are relevant as they allow translation to be initiated differently in eukaryotes.
  • Joe Hanson 16:34, 3 March 2012 (EST):Could you add something about Internal Ribosome Entry Sites (IRES)? Those are kind of neat, they fake out the ribosome using RNA structure, usually from viruses like EMCV.
  • Michael Hammerling 16:57, 4 March 2012 (EST) It'd be nice to see a nod to alternative genetic codes and orthogonal ribosomes. Frances Arnold mentioned a really interesting project where they have evolved a ribosome to decode a quadruplet genetic code. [1] I think our ability to actually use this ribosome in designed systems for a useful purpose is a ways away, however.
  1. Neumann H, Wang K, Davis L, Garcia-Alai M, and Chin JW. . pmid:20154731. PubMed HubMed [Neuman2010]
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