Talk:CH391L/S12/Unnatural Amino Acids: Difference between revisions
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*'''[[User:Yi Kou|Yi Kou]] 12:26, 1 April 2012 (EDT)''':why is that the 6 red of GFP (presence of amber codon) is dimmer than that of the counter part? | *'''[[User:Yi Kou|Yi Kou]] 12:26, 1 April 2012 (EDT)''':why is that the 6 red of GFP (presence of amber codon) is dimmer than that of the counter part? | ||
*'''[[User:Michael Hammerling|Michael Hammerling]] 18:51, 1 April 2012 (EDT)''' I'm not sure you can make the conclusion that Red 6 is dimmer than Black 6 in that figure. It doesn't look dimmer to me. They do not indicate in the paper that this is the case. | |||
*'''[[User:Jeffrey E. Barrick|Jeffrey E. Barrick]] 17:30, 1 April 2012 (EDT)''':Add a note about why only 50 aa peptides can be synthesized, when oligos of > 100 nt can be synthesized? | *'''[[User:Jeffrey E. Barrick|Jeffrey E. Barrick]] 17:30, 1 April 2012 (EDT)''':Add a note about why only 50 aa peptides can be synthesized, when oligos of > 100 nt can be synthesized? | ||
*'''[[User:Michael Hammerling|Michael Hammerling]] 18:51, 1 April 2012 (EDT)''' Done | |||
*'''[[User:Jeffrey E. Barrick|Jeffrey E. Barrick]] 17:30, 1 April 2012 (EDT)''':Add citation for native chemical ligation figure. | *'''[[User:Jeffrey E. Barrick|Jeffrey E. Barrick]] 17:30, 1 April 2012 (EDT)''':Add citation for native chemical ligation figure. | ||
*'''[[User:Michael Hammerling|Michael Hammerling]] 18:51, 1 April 2012 (EDT)''' Done | |||
Revision as of 15:51, 1 April 2012
- James L. Bachman 12:54, 29 March 2012 (EDT): Has it been shown that incorporation of UAAs can confer an advantageous phenotype in engineered cells? If not, what a promising example of an engineered cell using UAAs in a way that it is more beneficial than using natural AA's?
- Jeffrey E. Barrick 17:30, 1 April 2012 (EDT):Are there any studies that use UAA in directed evolution to show an advantage to having the UAA? I know that there are various studies that introduce new chemical functionalities into RNAs during in vitro selection and that they are able to show that they get better function with things like histidines incorporated into the RNA.
- Yi Kou 12:26, 1 April 2012 (EDT):why is that the 6 red of GFP (presence of amber codon) is dimmer than that of the counter part?
- Michael Hammerling 18:51, 1 April 2012 (EDT) I'm not sure you can make the conclusion that Red 6 is dimmer than Black 6 in that figure. It doesn't look dimmer to me. They do not indicate in the paper that this is the case.
- Jeffrey E. Barrick 17:30, 1 April 2012 (EDT):Add a note about why only 50 aa peptides can be synthesized, when oligos of > 100 nt can be synthesized?
- Michael Hammerling 18:51, 1 April 2012 (EDT) Done
- Jeffrey E. Barrick 17:30, 1 April 2012 (EDT):Add citation for native chemical ligation figure.
- Michael Hammerling 18:51, 1 April 2012 (EDT) Done
