Talk:CH391L/S13/DirectedProteinEvolution

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*'''[[User:Kevin Baldridge|Kevin Baldridge]] 16:43, 25 February 2013 (EST)''':Gotta give a plug for the technique my group likes [http://www.sciencedirect.com/science/article/pii/S0022283607008790 secM ribosome display]
*'''[[User:Kevin Baldridge|Kevin Baldridge]] 16:43, 25 February 2013 (EST)''':Gotta give a plug for the technique my group likes [http://www.sciencedirect.com/science/article/pii/S0022283607008790 secM ribosome display]
*'''[[User:Kevin Baldridge|Kevin Baldridge]] 16:44, 25 February 2013 (EST)''':it would be nice to have a one or two sentence summary of the other techniques you list in the advanced/high-throughput section
*'''[[User:Kevin Baldridge|Kevin Baldridge]] 16:44, 25 February 2013 (EST)''':it would be nice to have a one or two sentence summary of the other techniques you list in the advanced/high-throughput section
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*'''[[User:Gabriel Wu|Gabriel Wu]] 16:55, 25 February 2013 (EST)''':  For those of you who do this sort of thing, have you any thoughts on the contexts in which Directed evolution works well and, more importantly, where it doesn't work well? Since most papers are about successes, do you know of any examples (personal communication/experience) where it doesn't work and speculation on why?

Revision as of 17:55, 25 February 2013

  • Alvaro E. Rodriguez M. 21:45, 21 February 2013 (EST):It would be nice if you added literature examples of Directed Evolution of Proteins for each approach as done in the Ancestral Sequence Reconstruction or take a similar approach.
  • Kevin Baldridge 16:43, 25 February 2013 (EST):Gotta give a plug for the technique my group likes secM ribosome display
  • Kevin Baldridge 16:44, 25 February 2013 (EST):it would be nice to have a one or two sentence summary of the other techniques you list in the advanced/high-throughput section
  • Gabriel Wu 16:55, 25 February 2013 (EST): For those of you who do this sort of thing, have you any thoughts on the contexts in which Directed evolution works well and, more importantly, where it doesn't work well? Since most papers are about successes, do you know of any examples (personal communication/experience) where it doesn't work and speculation on why?
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