Talk:CH391L/S13/GeneticMarkers: Difference between revisions
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Gabriel Wu (talk | contribs) (New page: *'''~~~~''': On a practical note, ampicillin (amp), kanamycin (kan), and chloramphenicol (cam) are the most used antibiotic selection markers in synthetic biology. Amp in my experience is...) |
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*'''[[User:Gabriel Wu|Gabriel Wu]] 17:30, 4 March 2013 (EST)''': On a practical note, ampicillin (amp), kanamycin (kan), and chloramphenicol (cam) are the most used antibiotic selection markers in synthetic biology. Amp in my experience is convenient, but somewhat "fragile" (relative to kan and cam). Convenient: typically "rescuing" your cells post-transformation is unnecessary when using amp. "Rescuing" is necessary for kan and cam. Cam and kan are useful for long time course experiments (longer than 24 hours) because amp generally breaks down (or is broken down) over time. | *'''[[User:Gabriel Wu|Gabriel Wu]] 17:30, 4 March 2013 (EST)''': On a practical note, ampicillin (amp), kanamycin (kan), and chloramphenicol (cam) are the most used antibiotic selection markers in synthetic biology. Amp in my experience is convenient, but somewhat "fragile" (relative to kan and cam). Convenient: typically "rescuing" your cells post-transformation is unnecessary when using amp. "Rescuing" is necessary for kan and cam. Cam and kan are useful for long time course experiments (longer than 24 hours) because amp generally breaks down (or is broken down) over time. | ||
*'''[[User:Gabriel Wu|Gabriel Wu]] 17:33, 4 March 2013 (EST)''': For those who work outside of E. coli, there's a class of selection markers used more commonly in yeast known as auxotrophic markers. Maybe not this time, but this topic should be addressed on this page at some point. | |||
Revision as of 15:33, 4 March 2013
- Gabriel Wu 17:30, 4 March 2013 (EST): On a practical note, ampicillin (amp), kanamycin (kan), and chloramphenicol (cam) are the most used antibiotic selection markers in synthetic biology. Amp in my experience is convenient, but somewhat "fragile" (relative to kan and cam). Convenient: typically "rescuing" your cells post-transformation is unnecessary when using amp. "Rescuing" is necessary for kan and cam. Cam and kan are useful for long time course experiments (longer than 24 hours) because amp generally breaks down (or is broken down) over time.
- Gabriel Wu 17:33, 4 March 2013 (EST): For those who work outside of E. coli, there's a class of selection markers used more commonly in yeast known as auxotrophic markers. Maybe not this time, but this topic should be addressed on this page at some point.
