Talk:CH391L/S13/Metabolic Engineering and Thermophiles

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  • Gabriel Wu 17:11, 15 April 2013 (EDT): The latest and greatest on the artemisinin project.
  • Dwight Tyler Fields 17:31, 15 April 2013 (EDT): From a big picture perspective, what are the boundaries on what one can get cells to produce? Maybe you could include this in a brief section on "future challenges" in the field?
  • Evan Weaver 13:04, 16 April 2013 (EDT): What makes thermophiles so hard to culture? Is it because of the high heat in combination with the amount of pressure needed to keep them alive?
    • Aurko Dasgupta 18:44, 16 April 2013 (EDT):I think thermophiles typically have a slower growth rate that mesotherms like E. coli. Deinococcus radiodurans (not a thermophile, but another kind of extremophile) takes 2-3 days to form decent sized colonies on plates. I think the difficulty of transformation is also partially due to differences in their exponential phase compared to normal bacteria.
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