Talk:CH391L/S13/Riboswitches

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*'''[[User:Catherine I. Mortensen|Catherine I. Mortensen]] 13:45, 25 February 2013 (EST)''': How practical is DNT detecting E. Coli in airports and other public areas considering the time it takes for E. Coli to start producing fluorescent protein?
*'''[[User:Catherine I. Mortensen|Catherine I. Mortensen]] 13:45, 25 February 2013 (EST)''': How practical is DNT detecting E. Coli in airports and other public areas considering the time it takes for E. Coli to start producing fluorescent protein?
*'''[[User:Kevin Baldridge|Kevin Baldridge]] 17:08, 25 February 2013 (EST)''':Are there any known examples of a riboswitch with an IRES (Internal Ribosome Entry Site, an alternate way to initiate translation that doesn't use the Shine Dalgarno sequence) expression platform? If not, that might be something we could look into for iGEM, directed evolution or SELEX to design such a system.
*'''[[User:Kevin Baldridge|Kevin Baldridge]] 17:08, 25 February 2013 (EST)''':Are there any known examples of a riboswitch with an IRES (Internal Ribosome Entry Site, an alternate way to initiate translation that doesn't use the Shine Dalgarno sequence) expression platform? If not, that might be something we could look into for iGEM, directed evolution or SELEX to design such a system.
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**'''[[User:Catherine I. Mortensen|Catherine I. Mortensen]] 08:59, 26 February 2013 (EST)''':What would be the advantage of IRES as opposed to a Shine Dalgarno?
*'''[[User:Gabriel Wu|Gabriel Wu]] 17:17, 25 February 2013 (EST)''': Connecting with Max's topic, directed evolution strategies are very relevant in  developing riboswitches, especially for aptamer design. Might be nice to discuss the connection between the two topics.
*'''[[User:Gabriel Wu|Gabriel Wu]] 17:17, 25 February 2013 (EST)''': Connecting with Max's topic, directed evolution strategies are very relevant in  developing riboswitches, especially for aptamer design. Might be nice to discuss the connection between the two topics.
*'''[[User:Kevin Baldridge|Kevin Baldridge]] 17:18, 25 February 2013 (EST)''':Here is a nice example of taking advantage of riboswitch concepts for synthetic biology [http://www.nature.com/nbt/journal/v22/n7/abs/nbt986.html Isaacs 2004], our group is working on developing a probe for ''in vivo'' analysis of RNA tertiary structure
*'''[[User:Kevin Baldridge|Kevin Baldridge]] 17:18, 25 February 2013 (EST)''':Here is a nice example of taking advantage of riboswitch concepts for synthetic biology [http://www.nature.com/nbt/journal/v22/n7/abs/nbt986.html Isaacs 2004], our group is working on developing a probe for ''in vivo'' analysis of RNA tertiary structure
*'''[[User:Kevin Baldridge|Kevin Baldridge]] 17:26, 25 February 2013 (EST)''':it looks like your biblio section might have a few of the entries merged together at the end?
*'''[[User:Kevin Baldridge|Kevin Baldridge]] 17:26, 25 February 2013 (EST)''':it looks like your biblio section might have a few of the entries merged together at the end?
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**'''[[User:Catherine I. Mortensen|Catherine I. Mortensen]] 08:59, 26 February 2013 (EST)''': Thank you! Fixed.

Revision as of 08:59, 26 February 2013

  • Catherine I. Mortensen 13:45, 25 February 2013 (EST): How practical is DNT detecting E. Coli in airports and other public areas considering the time it takes for E. Coli to start producing fluorescent protein?
  • Kevin Baldridge 17:08, 25 February 2013 (EST):Are there any known examples of a riboswitch with an IRES (Internal Ribosome Entry Site, an alternate way to initiate translation that doesn't use the Shine Dalgarno sequence) expression platform? If not, that might be something we could look into for iGEM, directed evolution or SELEX to design such a system.
    • Catherine I. Mortensen 08:59, 26 February 2013 (EST):What would be the advantage of IRES as opposed to a Shine Dalgarno?
  • Gabriel Wu 17:17, 25 February 2013 (EST): Connecting with Max's topic, directed evolution strategies are very relevant in developing riboswitches, especially for aptamer design. Might be nice to discuss the connection between the two topics.
  • Kevin Baldridge 17:18, 25 February 2013 (EST):Here is a nice example of taking advantage of riboswitch concepts for synthetic biology Isaacs 2004, our group is working on developing a probe for in vivo analysis of RNA tertiary structure
  • Kevin Baldridge 17:26, 25 February 2013 (EST):it looks like your biblio section might have a few of the entries merged together at the end?
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