Talk:Endy:Preparing Antibiotic Stocks: Difference between revisions

Revision as of 06:08, 17 January 2008

Chloramphenicol

   * Stock concentration - 34mg/ml in 100% Ethanol
   * Aliquots - 1ml
   * Working concentration = 25μ/ml (Stringent), 170μ/ml (relaxed)

Are stringent and relaxed the wrong way around...?

Jason R. Kelly 07:08, 17 January 2008 (CST): I think it's right. Relaxed plasmids are normally higher copy, so you'd expect a higher concentration of antibiotic for them.

Revision as of 05:31, 17 January 2008 (view source) Hugh Kingston (talk \| contribs) (New page: Chloramphenicol * Stock concentration - 34mg/ml in 100% Ethanol * Aliquots - 1ml * Working concentration = 25μ/ml (Stringent), 170μ/ml (relaxed) Are stringent and relaxed...)		Revision as of 06:08, 17 January 2008 (view source) Jason R. Kelly (talk \| contribs) No edit summary Newer edit →
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	Are stringent and relaxed the wrong way around...?		Are stringent and relaxed the wrong way around...?
			*'''[[User:Jason R. Kelly\|Jason R. Kelly]] 07:08, 17 January 2008 (CST):''' I think it's right. Relaxed plasmids are normally higher copy, so you'd expect a higher concentration of antibiotic for them.