Talk:Endy:Preparing Antibiotic Stocks

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Are stringent and relaxed the wrong way around...?
Are stringent and relaxed the wrong way around...?
*'''[[User:Jason R. Kelly|Jason R. Kelly]] 07:08, 17 January 2008 (CST):''' I think it's right.  Relaxed plasmids are normally higher copy, so you'd expect a higher concentration of antibiotic for them.
*'''[[User:Jason R. Kelly|Jason R. Kelly]] 07:08, 17 January 2008 (CST):''' I think it's right.  Relaxed plasmids are normally higher copy, so you'd expect a higher concentration of antibiotic for them.
 +
*'''[[User:Hugh Kingston|hugh kingston]] 10:16, 4 February 2008 (CST)''': ok. i did not realise stringent and relaxed were types of plasmid, rather i thought the words referred to the strength of selection for the marker.

Revision as of 12:16, 4 February 2008

Chloramphenicol
   * Stock concentration - 34mg/ml in 100% Ethanol
   * Aliquots - 1ml
   * Working concentration = 25μ/ml (Stringent), 170μ/ml (relaxed) 

Are stringent and relaxed the wrong way around...?

  • Jason R. Kelly 07:08, 17 January 2008 (CST): I think it's right. Relaxed plasmids are normally higher copy, so you'd expect a higher concentration of antibiotic for them.
  • hugh kingston 10:16, 4 February 2008 (CST): ok. i did not realise stringent and relaxed were types of plasmid, rather i thought the words referred to the strength of selection for the marker.
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