Talk:Endy:Preparing Antibiotic Stocks
From OpenWetWare
(Difference between revisions)
Current revision (12:16, 4 February 2008) (view source) |
|||
| Line 7: | Line 7: | ||
Are stringent and relaxed the wrong way around...? | Are stringent and relaxed the wrong way around...? | ||
*'''[[User:Jason R. Kelly|Jason R. Kelly]] 07:08, 17 January 2008 (CST):''' I think it's right. Relaxed plasmids are normally higher copy, so you'd expect a higher concentration of antibiotic for them. | *'''[[User:Jason R. Kelly|Jason R. Kelly]] 07:08, 17 January 2008 (CST):''' I think it's right. Relaxed plasmids are normally higher copy, so you'd expect a higher concentration of antibiotic for them. | ||
| + | *'''[[User:Hugh Kingston|hugh kingston]] 10:16, 4 February 2008 (CST)''': ok. i did not realise stringent and relaxed were types of plasmid, rather i thought the words referred to the strength of selection for the marker. | ||
Current revision
Chloramphenicol
* Stock concentration - 34mg/ml in 100% Ethanol * Aliquots - 1ml * Working concentration = 25μ/ml (Stringent), 170μ/ml (relaxed)
Are stringent and relaxed the wrong way around...?
- Jason R. Kelly 07:08, 17 January 2008 (CST): I think it's right. Relaxed plasmids are normally higher copy, so you'd expect a higher concentration of antibiotic for them.
- hugh kingston 10:16, 4 February 2008 (CST): ok. i did not realise stringent and relaxed were types of plasmid, rather i thought the words referred to the strength of selection for the marker.
