Talk:IGEM:IMPERIAL/2007/Projects/In-Veso/Presentations/31-08-07: Difference between revisions
(New page: hey dirk just a few comments - Specs: * people don't like the words toxic and hazardous (although we are dealing with hydrocarbons) * could do with a prioritised list of specifications * ...) |
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Where does in veso gene expression come in? | Where does in veso gene expression come in? | ||
Vesicles are a means whereby we can attempt to circumvent some of the problems that we face with the inherent characteristics of the in vitro system (eg. lifespan). it also provides an alternative platform to in vitro expression in our promotion of cell-free systems. While in vitro systems would no doubt be the main platform for our application, the future of cell-free systems lie in the possiblity of creating an artificial environment where we can control gene expression, and indeed, engineer biology. | Vesicles are a means whereby we can attempt to circumvent some of the problems that we face with the inherent characteristics of the in vitro system (eg. lifespan). it also provides an alternative platform to in vitro expression in our promotion of cell-free systems. While in vitro systems would no doubt be the main platform for our application, the future of cell-free systems lie in the possiblity of creating an artificial environment where we can control gene expression, and indeed, engineer biology. | ||
The selling point of vesicles | The selling point of vesicles | ||
If we choose to pursue vesicle formations, they no doubt will probably not contribute to our applications in time for the jamboree. however with respect to the selling point, and the promotion of the idea of cell free systems as an alternative chassis to bacteria, being able to qualitatively demonstrate the potential functionability of vesicles gives us the oppurtunity to capture the imagination of the audience as we discuss the future of cell free systems. having expanded the realms of applications in synthetic biology, we are not only breaking barriers in terms of application, but are also attempting to pursue a prototype gene expression machine where we can actually design what goes into the machinery, as well as incorporate manufacturing and production methods (like a production assembly). | If we choose to pursue vesicle formations, they no doubt will probably not contribute to our applications in time for the jamboree. however with respect to the selling point, and the promotion of the idea of cell free systems as an alternative chassis to bacteria, being able to qualitatively demonstrate the potential functionability of vesicles gives us the oppurtunity to capture the imagination of the audience as we discuss the future of cell free systems. having expanded the realms of applications in synthetic biology, we are not only breaking barriers in terms of application, but are also attempting to pursue a prototype gene expression machine where we can actually design what goes into the machinery, as well as incorporate manufacturing and production methods (like a production assembly). | ||
Revision as of 15:14, 30 August 2007
hey dirk just a few comments -
Specs:
- people don't like the words toxic and hazardous (although we are dealing with hydrocarbons)
- could do with a prioritised list of specifications
- AHL, being a small molecule, maybe able to diffuse in and out of the vesicle (at last thats for E. coli)
Cell by Date
1. Be functional and maintain a constant (or predictable) population for at least 4 days
2. Be functional and maintain a constant (or predictable) population in temperatures between 4°C and 37°C.
3. Be permeable to nutrients required for gene expression
Infector Detector
1. Be permeable to AHL???
2. Be able to produce a visible FP signal within 3 hours
What needs to be done:
- Nice!
Work done to date:
- Pictures?
- empty vesicle formation + use of various lipids and oils
the rest are all fine...
Recommendation (Alex)
In relation to the competition, our team currently has the storyline of detecting stuff, and is showcasing the use of cell-free systems that would allow us to achieve the specifications of our applications, which we were previously unable to due to the limitations of bacteria. Our edge in the competition therefore lies in that we are able to expand the applicative nature of synthetic biology because of our new chassis.
The preliminary results of in vitro systems have so far demonstrated its versatality and its limitations, and not all the specifications required by the applications have been met with in vitro translation. This means that despite the efforts of the team, we are still falling short in terms of the specifications that is required for our applications - something that we are finding solutions to and may not simply due to the inherent characteristics of the system itself.
Where does in veso gene expression come in?
Vesicles are a means whereby we can attempt to circumvent some of the problems that we face with the inherent characteristics of the in vitro system (eg. lifespan). it also provides an alternative platform to in vitro expression in our promotion of cell-free systems. While in vitro systems would no doubt be the main platform for our application, the future of cell-free systems lie in the possiblity of creating an artificial environment where we can control gene expression, and indeed, engineer biology.
The selling point of vesicles
If we choose to pursue vesicle formations, they no doubt will probably not contribute to our applications in time for the jamboree. however with respect to the selling point, and the promotion of the idea of cell free systems as an alternative chassis to bacteria, being able to qualitatively demonstrate the potential functionability of vesicles gives us the oppurtunity to capture the imagination of the audience as we discuss the future of cell free systems. having expanded the realms of applications in synthetic biology, we are not only breaking barriers in terms of application, but are also attempting to pursue a prototype gene expression machine where we can actually design what goes into the machinery, as well as incorporate manufacturing and production methods (like a production assembly).
Im sorry with the recommendations bit. got carried away writing. please edit freely.
cheers, alex
