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| *[http://mts-msb.nature.com/cgi-bin/main.plex?form_type=display_auth_instructions Author guidlines for Nature MSB] | | *[http://mts-msb.nature.com/cgi-bin/main.plex?form_type=display_auth_instructions Author guidlines for Nature MSB] |
| *[http://www.pnas.org/misc/iforc.shtml PNAS information for authors] | | *[http://www.pnas.org/misc/iforc.shtml PNAS information for authors] |
| | *[http://www.pnas.org/misc/LaTex.shtml Latex template] |
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| | ==General outstanding issues== |
| | *Make sure J45900 gets into registry |
| | *Submit sequences to Genbank |
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| ==Questions for Stephen== | | ===Odor thresholds=== |
| #Is the sequence of the J45017 reverse primer correct? It seems quite short.
| | "The odor threshold of a compound is the lowest concentration at which its smell can be detected." |
| #*A: I think I checked both the tube and the wiki, but I can doublecheck tomorrow.
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| #Did you use the Knight or Endy site directed mutagenesis protocol? (http://openwetware.org/wiki/IGEM:MIT/2006/Notebook/2006-8-10#Mutagenesis says Knight but you told me Endy)
| | *[http://people.ok.ubc.ca/neggers/Chem422A/The%20Odor%20and%20Aroma%20of%20Wine.pdf Isoamyl acetate] |
| #*A: We used the Knight protocol.
| | *[http://www.sciencelab.com/xMSDS-Methyl_salicylate-9927362 Methyl salicylate] |
| #Quantification of banana timecourse
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| #*A: I sent both Alex and Amy an email today about a meeting tomorrow.
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| #**You might want to do some background research on quantification of GC data.
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| #The reverse primer for <bbpart>BBa_J45992</bbpart> listed in the google doc does not match what is listed in the Registry
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| #*A: I will double check this, but I'm pretty sure that I confirmed this via the wiki and Isadora's order sheets. I do not know who wrote the Registry page (maybe Andre), but I do trust the lab notebook more than the Registry.
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| #**OK then the Registry page needs to be fixed after you confirm.
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| #***Double-checked... google doc is correct, fixed Registry
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| #The forward and reverse primers for <bbpart>BBa_J45993</bbpart> listed in the google doc does not match what is listed in the Registry
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| #*A: I will double check this, but I'm pretty sure that I confirmed this via the wiki and Isadora's order sheets. I do not know who wrote the Registry page (maybe Andre), but I do trust the lab notebook more than the Registry.
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| #**OK then the Registry page needs to be fixed after you confirm.
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| #***Double-checked... google doc is correct, fixed Registry
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| #BBa_J45900 needs to be entered into the Registry
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| #<bbpart>BBa_J45992</bbpart> and <bbpart>BBa_J45994</bbpart> need to be submitted to the Registry. Or at least the GFP versions do.
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| #Does <bbpart>BBa_J45017</bbpart> still have the PstI site or has it been mutated out? If it has been mutated out, then the Registry needs to be updated.
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