Talk:IGEM:MIT/2006/Communications: Difference between revisions

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*[http://mts-msb.nature.com/cgi-bin/main.plex?form_type=display_auth_instructions Author guidlines for Nature MSB]
*[http://mts-msb.nature.com/cgi-bin/main.plex?form_type=display_auth_instructions Author guidlines for Nature MSB]
*[http://www.pnas.org/misc/iforc.shtml PNAS information for authors]
*[http://www.pnas.org/misc/iforc.shtml PNAS information for authors]
*[http://www.pnas.org/misc/LaTex.shtml Latex template]


==General outstanding issues==
*Make sure J45900 gets into registry
*Submit sequences to Genbank


==Questions for Stephen==
===Odor thresholds===
#Did you use the Knight or Endy site directed mutagenesis protocol? (http://openwetware.org/wiki/IGEM:MIT/2006/Notebook/2006-8-10#Mutagenesis says Knight but you told me Endy)
"The odor threshold of a compound is the lowest concentration at which its smell can be detected." 
#*A: We used the Knight protocol.
 
#**Then are you sure that you phosphorylated the primers?  The Knight protocol doesn't call for this step.
*[http://people.ok.ubc.ca/neggers/Chem422A/The%20Odor%20and%20Aroma%20of%20Wine.pdf Isoamyl acetate]
#Quantification of banana timecourse
*[http://www.sciencelab.com/xMSDS-Methyl_salicylate-9927362 Methyl salicylate]
#*A: I sent both Alex and Amy an email today about a meeting tomorrow.
#**You might want to do some background research on quantification of GC data.
#BBa_J45900 needs to be entered into the Registry
#Does <bbpart>BBa_J45017</bbpart> still have the PstI site or has it been mutated out?  If it has been mutated out, then the Registry needs to be updated.
#Update the sequence of <bbpart>BBa_J45009</bbpart> to reflect the fact that BioBrick sites weren't eliminated.

Latest revision as of 12:59, 28 April 2008

General outstanding issues

  • Make sure J45900 gets into registry
  • Submit sequences to Genbank

Odor thresholds

"The odor threshold of a compound is the lowest concentration at which its smell can be detected."

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