Talk:IGEM:MIT/2006/Communications: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
 
(24 intermediate revisions by 2 users not shown)
Line 1: Line 1:
*[http://mts-msb.nature.com/cgi-bin/main.plex?form_type=display_auth_instructions Author guidlines for Nature MSB]
*[http://mts-msb.nature.com/cgi-bin/main.plex?form_type=display_auth_instructions Author guidlines for Nature MSB]
*[http://www.pnas.org/misc/iforc.shtml PNAS information for authors]
*[http://www.pnas.org/misc/iforc.shtml PNAS information for authors]
*[http://www.pnas.org/misc/LaTex.shtml Latex template]


==General outstanding issues==
*Make sure J45900 gets into registry
*Submit sequences to Genbank


==Questions for Stephen==
===Odor thresholds===
#Did you use the Knight or Endy site directed mutagenesis protocol? (http://openwetware.org/wiki/IGEM:MIT/2006/Notebook/2006-8-10#Mutagenesis says Knight but you told me Endy)
"The odor threshold of a compound is the lowest concentration at which its smell can be detected."  
#*A: We used the Knight protocol.
#**Then are you sure that you phosphorylated the primers?  The Knight protocol doesn't call for this step.
*I'm sure we did.
#Do we have the regulated wintergreen GC data in a form that we could generate plots similar to the banana timecourse?
*Yes, we could.  It would take some digging through old files and sorting out through each protocol we used on each day.  I don't know if it's necessary to show though.
#What did you use to shake the cultures? i.e. what incubator or shaker?
 
220 RPM- the shaker in 37 C room
 
110 RPM- the shaker by Samantha's/2007 iGEM team's bench
 
==General outstanding issues==
*Funding sources for each person
#See last email... all undergrads were funded in the summer of 2006 by the MIT UROP office
*Protocol for transformation
See below


#Transformation - André [http://openwetware.org/wiki/Transforming_chemically_competent_cells_%28Inoue%29 Transform competent cells]
*[http://people.ok.ubc.ca/neggers/Chem422A/The%20Odor%20and%20Aroma%20of%20Wine.pdf Isoamyl acetate]
#*Thaw ~50 μl cells (whichever you like) on ice. Do not use glass tubes, which adsorb DNA.
*[http://www.sciencelab.com/xMSDS-Methyl_salicylate-9927362 Methyl salicylate]
#*Add 200 uL DNA to cells
#*Incubate on ice for 30 minutes
#*Incubate cells for 50-60 seconds at 42°C.
#*Incubate cells on ice for 2 min.
#*Add 200-300μL of room temperature [[SOC]] (not critical)
#*Incubate for 1 hour at 37°C on shaker.
#*Spread 200 μl onto plates made with appropriate antibiotic.
#*Grow overnight at 37°C.
*Describing figure 6
*Update <bbpart>BBa_Q04401</bbpart>

Latest revision as of 12:59, 28 April 2008

General outstanding issues

  • Make sure J45900 gets into registry
  • Submit sequences to Genbank

Odor thresholds

"The odor threshold of a compound is the lowest concentration at which its smell can be detected."

Retrieved from "https://openwetware.org/mediawiki/index.php?title=Talk:IGEM:MIT/2006/Communications&oldid=200452"