Talk:IGEM:MIT/2006/Communications: Difference between revisions

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*[http://mts-msb.nature.com/cgi-bin/main.plex?form_type=display_auth_instructions Author guidlines for Nature MSB]
*[http://mts-msb.nature.com/cgi-bin/main.plex?form_type=display_auth_instructions Author guidlines for Nature MSB]
*[http://www.pnas.org/misc/iforc.shtml PNAS information for authors]
*[http://www.pnas.org/misc/iforc.shtml PNAS information for authors]
*[http://www.pnas.org/misc/LaTex.shtml Latex template]


==General outstanding issues==
*Make sure J45900 gets into registry
*Submit sequences to Genbank


==Questions for Stephen==
===Odor thresholds===
#Do we have the regulated wintergreen GC data in a form that we could generate plots similar to the banana timecourse?
"The odor threshold of a compound is the lowest concentration at which its smell can be detected."  
#*Yes, we could.  It would take some digging through old files and sorting out through each protocol we used on each day.  I don't know if it's necessary to show though.
#**Drew would like to show these data if we can.
#What did you use to shake the cultures? i.e. what incubator or shaker?
#*220 RPM- the shaker in 37 C room
#*110 RPM- the shaker by Samantha's/2007 iGEM team's bench
#Are both J45200 and J45250 on pSB1AT3?
#What concentration salicylic acid and isoamyl alcohol did we use for the scent generator tests?  Was it always 440 ppm isoamyl alcohol?
#A summary of your analysis of fixing the wintergreen precursor production would be useful


==General outstanding issues==
*[http://people.ok.ubc.ca/neggers/Chem422A/The%20Odor%20and%20Aroma%20of%20Wine.pdf Isoamyl acetate]
*Funding sources for each person
*[http://www.sciencelab.com/xMSDS-Methyl_salicylate-9927362 Methyl salicylate]
**See last email... all undergrads were funded in the summer of 2006 by the MIT UROP office
*Protocol for transformation
**Transformation - André [http://openwetware.org/wiki/Transforming_chemically_competent_cells_%28Inoue%29 Transform competent cells]
***Thaw ~50 μl cells (whichever you like) on ice. Do not use glass tubes, which adsorb DNA.
***Add 200 uL DNA to cells
***Incubate on ice for 30 minutes
***Incubate cells for 50-60 seconds at 42°C.
***Incubate cells on ice for 2 min.
***Add 200-300μL of room temperature [[SOC]] (not critical)
***Incubate for 1 hour at 37°C on shaker.
***Spread 200 μl onto plates made with appropriate antibiotic.
***Grow overnight at 37°C.
*Describing figure 6
*Update <bbpart>BBa_Q04401</bbpart>

Latest revision as of 12:59, 28 April 2008

General outstanding issues

  • Make sure J45900 gets into registry
  • Submit sequences to Genbank

Odor thresholds

"The odor threshold of a compound is the lowest concentration at which its smell can be detected."

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