Talk:IGEM:MIT/2006/Communications

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Questions for Stephen)
Line 4: Line 4:
==Questions for Stephen==
==Questions for Stephen==
-
#Is the sequence of the J45017 reverse primer correct?  It seems quite short.
+
#Is the sequence of the J45017 reverse primer correct?  It seems quite short
 +
A: I think I checked both the tube and the wiki, but I can doublecheck tomorrow.
#Did you use the Knight or Endy site directed mutagenesis protocol? (http://openwetware.org/wiki/IGEM:MIT/2006/Notebook/2006-8-10#Mutagenesis says Knight but you told me Endy)
#Did you use the Knight or Endy site directed mutagenesis protocol? (http://openwetware.org/wiki/IGEM:MIT/2006/Notebook/2006-8-10#Mutagenesis says Knight but you told me Endy)
 +
A: We used the Knight protocol.
#Quantification of banana timecourse
#Quantification of banana timecourse
 +
A: I sent both Alex and Amy an email today about a meeting tomorrow.
#You say in your eau d'e coli summary that the BAMT enzyme didn't produce a detectable smell but in the registry, this enzyme is listed as working (<bbpart>BBa_J45002</bbpart>)
#You say in your eau d'e coli summary that the BAMT enzyme didn't produce a detectable smell but in the registry, this enzyme is listed as working (<bbpart>BBa_J45002</bbpart>)
 +
A: It isn't working.
#The reverse primer for <bbpart>BBa_J45992</bbpart> listed in the google doc does not match what is listed in the Registry
#The reverse primer for <bbpart>BBa_J45992</bbpart> listed in the google doc does not match what is listed in the Registry
 +
A: I will double check this, but I'm pretty sure that I confirmed this via the wiki and Isadora's order sheets.  I do not know who wrote the Registry page (maybe Andre), but I do trust the lab notebook more than the Registry.
#The forward and reverse primers for <bbpart>BBa_J45993</bbpart> listed in the google doc does not match what is listed in the Registry
#The forward and reverse primers for <bbpart>BBa_J45993</bbpart> listed in the google doc does not match what is listed in the Registry
 +
A: I will double check this, but I'm pretty sure that I confirmed this via the wiki and Isadora's order sheets.  I do not know who wrote the Registry page (maybe Andre), but I do trust the lab notebook more than the Registry.
#Need paper from reference below
#Need paper from reference below
-
 
+
A: I will search for this paper among the pile of stuff in the lab tomorrow.  If I cannot find it, I would ask Kate since I believe that she was the one that read this paper.
<biblio>
<biblio>
#Serino-MolGenet-1995 pmid=7500944
#Serino-MolGenet-1995 pmid=7500944
</biblio>
</biblio>

Revision as of 14:23, 3 February 2008


Questions for Stephen

  1. Is the sequence of the J45017 reverse primer correct? It seems quite short.

A: I think I checked both the tube and the wiki, but I can doublecheck tomorrow.

  1. Did you use the Knight or Endy site directed mutagenesis protocol? (http://openwetware.org/wiki/IGEM:MIT/2006/Notebook/2006-8-10#Mutagenesis says Knight but you told me Endy)

A: We used the Knight protocol.

  1. Quantification of banana timecourse

A: I sent both Alex and Amy an email today about a meeting tomorrow.

  1. You say in your eau d'e coli summary that the BAMT enzyme didn't produce a detectable smell but in the registry, this enzyme is listed as working (<bbpart>BBa_J45002</bbpart>)

A: It isn't working.

  1. The reverse primer for <bbpart>BBa_J45992</bbpart> listed in the google doc does not match what is listed in the Registry

A: I will double check this, but I'm pretty sure that I confirmed this via the wiki and Isadora's order sheets. I do not know who wrote the Registry page (maybe Andre), but I do trust the lab notebook more than the Registry.

  1. The forward and reverse primers for <bbpart>BBa_J45993</bbpart> listed in the google doc does not match what is listed in the Registry

A: I will double check this, but I'm pretty sure that I confirmed this via the wiki and Isadora's order sheets. I do not know who wrote the Registry page (maybe Andre), but I do trust the lab notebook more than the Registry.

  1. Need paper from reference below

A: I will search for this paper among the pile of stuff in the lab tomorrow. If I cannot find it, I would ask Kate since I believe that she was the one that read this paper.

  1. Serino L, Reimmann C, Baur H, Beyeler M, Visca P, and Haas D. . pmid:7500944. PubMed HubMed [Serino-MolGenet-1995]
Personal tools